Methnol Wastewater Fermentation To Produce Bacterial Cellulose And Its Membrance Application Research

In the static fermentation process of Acetobacter xylinum using methanol wastewater as carbon source, observing and studying the pH value,ORP value, methanol concentration, residual glucose concentration and cellulose yield variation. Methanol wastewater medium in the condition of at an initial PH of5.95, fermentation temperature30℃, after culturing10d, the average production of bacterial cellulose to glucose fermentation liquid58.2%. Combined with the analysis of water quality after fermentation, methanol degradation rate reached99.88%, which explaining Acetobacter xylinum can fermentation to produce bacterial cellulose by methanol wastewater, but synthetic methanol wastewater fermentation liquid substances on bacterial cellulose has certain inhibition.Determining the feasibility of bacterial cellulose in methanol wastewater, using Plackett-Burman test and response method the to optimize the fermentation process for methanol and methanol wastewater simulated wastewater respectively. In methanol simulated wastewater fermentation process, ethanol and propanol at significant level P<0.1can case has a positive effect on cellulose yield;However, Methanol and butanol shows a negative effect. Which explans methanol and butanol has inhibitory effect on the growth of microorganisms, ethanol and isopropyl alcohol can promote the growth of microorganisms in the methanol wastewater. In methanol wastewater fermentation process, glucose, peptone, agar and cultivation time at significant level P＜0.1can case has a positive effect on cellulose yield. And the yeast powder, citric acid and temperature shows negative effects. Through Minitab software analysis, the optimal fermentation process for methanol wastewater:Temperature of20℃, glucose concentration of20g/L, agar concentration is0.25g/L, peptone6.88g/L, yeast powder2.5g/L,5% inoculation, citric acid lg/L, culture time10d, disodium hydrogen phosphate2g/L, Potassium dihydrogen phosphate1g/L.With bacterial cellulose as a additive, using phase inversion method of polyethersulfone (PES)/bacterial cellulose (BC) blend membranes, when the blend ratio of PES/BC=1/3, the hydrophilicity, mechanical strength, retention, resistance to pollution, exhibits excellent performance. Combined with magnesium silicate of methylene blue adsorption on filter integration adsorbent and membrane separation technology. For the initial concentration of0.1g/L of methylene blue solution, when the adsorption time is240Min, the maximum amount of adsorbent is0.3g/100ml, the adsorption equilibrium is reached, the decolorization rate of methylene blue reached more than99%. Adsorbent filtering the recovered, as a raw material for colored paint can be used directly as the coating preparation, provides support for feasible process for dye in dyeing wastewater resource.