| Triethylamine(TEA) is an aliphatic amine. In the chemical production TEA was used as accessories. For example, TEA could be used as solvent in the purification in penicillin and tetracycline antibiotic production, also could be used as inhibitor in tetrafluoroethylene production. TEA was important raw material of cypermethrin, insect net and pesticide phosphamidon. TEA also could be used as surfactants, fungicides, preservatives high-energy fuel and liquid rock propellant. Recently, with the development of medicine and pesticide industry, TEA was wildly used, the environmental emissions was also increased too. In the process of production and use, TEA may become a kind of environmental pollutants and do harm to the bodies.A novel highly effective TEA-degrading bacterial strain, designated SYA-1, was isolated from an activated sludge used to treat pharmaceutical wastewater. The strain was identified as Achromobacter sp. using an analysis of its16S ribosomal RNA gene sequence (16S rRNA) and an examination of its morphological, physiological and biochemical properties. The strain SYA-1was confirmed by gas chromatography(GC) that it had good degradation for TEA. Physiological and biochemical identification of strain SYA-1was gram negative; gelatin liquefaction and catalase test positive; starch, V.P test and denitrification negative.The optimum growth temperature and pH was30℃and7.0, strain SYA-1also grew well in25-35℃and pH6.0-8.0; when the volume of liquid was in the rang of50-125mL, the strain grew well without inhibition; in all the carbon sources, the strain could highly utilize fructose and had a highest utilization of lactose.The strain consumed TEA as its sole carbon and nitrogen source, degrading200mg/L TEA to a non-detectable level within24h. The optimum degradation conditions were optimized as following:temperature30℃, initial pH7.0, NaCl≤10g/L; when the initial concentration of TEA was50-500mg/L, strain SYA-1could degrade completely in52h; Further study showed that heavy metal ions could inhibit the degradation of TEA in different extent, the inhibitory effect in a descending order was:Cu+>Co+>Ag+>Cd2+ >Fe3+>Pb2+.The results showed that the maximum specific growth rate was0.0574h-1for an initial TEA concentration of200mg/L. Four kinetic models (Monod, Haldane, Luong and Yano) were fitted to the experimental growth kinetic data. The Yano model was the most suitable model (R2=0.963) with values of μmax, Ks, Ki and K equal to0.099h-1,71mg/L,3228mg/L and36mg/L, respectively. These data provided basic data and theoretical basis for related research. The kinetics process in the degradation of TEA by strain SYA-1and strain EYA-2followed the Yano model, and the parameters were:μmax=0.091h-1; Ks=85mg/L; Ki=6600mg/L. The high value of Ki suggested that this strain SYA-1and strain EYA-2had a high tolerance for high TEA concentrations. This study showed that strain SYA-1and strain EYA-2were excellent candidates for the removal of TEA from aqueous solutions.In the engineering application, the strain SYA-1was added in SBR biochemical system, the influence of TEA wastewater treatment capacity and the ability to remove COD was studied. After a short time of acclimation, activated sludge with strain SYA-1could degrade TEA as sole carbon and nitrogen sources. The test provided theoretical basis for engineering application parameters such as influent concentration. |
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