| Magnetic nanoparticles binding with drugs were regarded as innovative,convenient, and highly efficient systems for drug delivery. It not only provide efficacyof drug but also break bottleneck of toxic side effects. Doxorubicin hydrochloride(DOX) is one of available drug for treating abroad range of cancers；Nevertheless, thecardiotoxicity limit its clinical application. A satisfactory solution is to design targetingdrugs, which not only deliver the drug to the tumor sites, but also protect normal cellsfrom the unwanted toxic side effects. In addition, Fe2O3nanoparticles have beenwidely used in drug delivery system, due to good biocompatibility and specificity.In this study, the magnetic nanoparticle of Fe2O3was synthesized by the chemicalco-precipitation method and modified with meso-2,3-dimercaptosuccinic acid (DMSA)to prevent the aggregation of nanoparticles. Doxorubicin hydrochloride (DOX) wasimmobilized on the surface of DMSA-Fe2O3nanoparticles by electrostatic attraction.DMSA-Fe2O3and DMSA-Fe2O3loading DOX (DMSA-Fe2O3@DOX) werecharacterized by Transmission Electron Microscopy (TEM), Vibrating SampleMagnetometer (VSM), Ultraviolet and Visible Spectrophotometer (UV), and FourierTransformed Infrared Spectroscopy (FTIR), respectively. The results indicated thatDOX can be loaded on the surface of nanoparticles successfully.The optimal conditionwas in a DOX concentration of17μg/mL, adsorption time for12h and pH of8.9inmagnetic nanoparticles suspension (1mg/mL), achieving the DOX loading rate as highas89%.At last, through anti-tumor experiments, It demonstrated that DMSA-Fe2O3didnot exhibit significant cytotoxic activity toward HepG2cells. The cytotoxicity toHepG2cells that treated with DMSA-Fe2O3@DOX was slightly higher than that ofDOX alone. |
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