The Application Of Cold Adapted Cellulase Producted By Antarctic Fungi QP8on Kelp Resource To Degrade And A Pilot Test Of Using Suaeda Salsa To Make Fuel Ethanol

In the context of global energy crisis, humans begin to work hard on looking fornew alternative energys. As a sunrise industry, cellulosic ethanol, ascendant, hasadvantages including big energy storage amount and renewable sources of rawmaterials, etc. In this paper, we take kelp residue, suaeda salsa as raw materials, studythe optimum conditions of converting them into ethanol. Through the small scaleenlarge production of suaeda salsa, we try to provide the previous basic data andtheoretical support for the industrialization development of synthesis using thissubstance to product fuel ethanol and other products.When combining dilute acid pretreatment and cellulose enzymolysis tosaccharificate kelp resource, we found the complex compositions in the pretreatmentsolution can effect the catalytic activity of cellulose. Through single factor analysis andthe response surface method optimization, we found that the dilute acid pretreatmentthat under the conditions of2.5%of sulfuric acid concentration,2.1h of acid solutiontime and121℃of solution temperature can keep cellulase catalytic ability effectively,the reducing sugar concentration reaches5.2g/100mL. The whole saccharificationprocesses degradate86%of cellulose and52%of hemicellulose of the raw material. Inkelp residue saccharification liquid, there are also so many substances that inhibit theethanol fermentation, such as formic acid, furfural, etc. by the detection of HPLC, itshows that the contents of2-F and5-HMF in the saccharification liquid up to132mg/L and158mg/L, of which2-F faint promotes the yeast fermentation but5-HMFmakes ethanol concentration after fermentation reduce by8.3%. Through a number ofdetoxification methods including extraction, evaporation and detoxification andadsorption, we found that using Ca(OH)2to adjust saccharification liquid pH to10andthen using sulfuric acid to neutralize can remove94.4%of2-F and77.7%5-HMF, thefinal fermentation ethanol concentration gets2.05%,10.9%more than the untreatedsamples.In addition, considering that single cellulase is incomplete of enzyme system,taking cold adapted cellulase producted by antarctic fungi QP8as the object, we studythe ability of the cellulase (crude enzyme powder) to enzymolysis kelp residue, theresult is not ideal, and then through compounding the cold adapted cellulose withcommercial enzyme, we found the optimum compound cellulose can make the reducing sugar concentration up to5.48%,5.4%more than the result that commercialenzyme works alone.Suaeda salsa is widely distributed on the coastal beach of china, the cellulose,hemicellulose in the dry weight raw material reaches36.1%and37.0%. We build asmall scale test line which covers about600m2to convert lignocellulose into ethanoland other substance by a specialized research fund. In the production process, we usesuaeda salsa as the raw material to product fuel ethanol and xylose (alcohol). Aftersmall scale test, we found120kg suaeda salsa in one production batch can convert into17.73L95%ethanol (about13.3kg) and16.20kg decolored Crude xylose powdercontaining78%xylose.