| Resonance Rayleigh scattering(RRS) is a new analytical technology developed in1990s. Due to its high sensitivity and remarkable characteristics of simple operation this method brings to more attention. At present, this method has been successfully used to determination and study of trace nonmetal ions, metal ions, biological macro molecules and nanoparticle probe et al. Furthermore, when ions of different charges form ion association complexes by hydrophobic force, electrostatic force and charge transferring effect, RRS can be enhanced.In this thesis, taking mebendazole and albendazole as research objects, we mainly investigated the interaction between12-tungstophosphoric acid, PdCl2, erythrosine and eosin Y by RRS method. The spectral characteristics, the optimum conditions of RRS method and the influence factors were discussed. The reaction mechanisms and the reasons for RRS enhancement of the systems were discussed.Under the supports of the National Natural Science Foundation of China (No.21175015), we studied the follow research systems:1. Resonance Rayleigh scattering, frequency doubling scattering and absorption spectra of the interaction of mebendazole with12-tungstophosphoric acid and their analytical applicationsThe interaction of mbendazole(MBZ) withl2-tungstophosphoric acid(TP) had been investigated by using resonance Rayleigh scattering(RRS) and frequency doubling scattering(FDS) combining with absorption spectrum. In HCl(pH=1.0) medium, MBZ reacted with TP to form ion-association complex(nMBz:nTP=3:1). In consequence, the spectrum of absorption was changed、the intensities of RRS and FDS were enhanced sharply. The maximum RRS wavelength was located at372nm, FDS wavelength was located at392nm and absorption wavelength was located at260nm. The increments of scattering intensity(△I) and absorption(△A) were directly proportional to the concentrations of MBZ in0.01-4.0ug/mL ranges. The detection limits(3σ) of RRS were0.56ng/mL, FDS were0.86ng/mL and absorption were130.16ng/mL. The sensitivity of RRS method was highest. In the paper, the optimum conditions of RRS method, the influence factors were discussed. Based on the ion-association reaction and its spectral response, the rapid, simple and sensitive RRS method for the determination of MBZ has been developed.2. Resconance Rayleigh scattering, second-order scattering and frequency doubling scattering spectra of mebendazole-palladium chloride system and its analytical applicationsWe present here resonance Rayleigh scattering(RRS)、second-order scattering(SOS) and frequency doubling scattering(FDS) methods for the determination of mebendazole(MBZ) at the nanogram level using palladium chloride for probe reagent. It was found that MBZ could react with Pd(Ⅱ) to form an ion-association complex, which induced the enhancement of RRS, SOS and FDS intensities in Britton-Robinson(BR)(pH=6.8～7.0)buffer medium. In certain ranges, the increments of scattering intensity(△I) were directly proportional to the concentrations of MBZ. The detection limits(3σ) of MBZ were1.55ng/mL(RRS),3.84ng/mL(SOS) and6.02ng/mL(FDS), separately. The sensitivity of RRS method was highest. The optimum conditions of RRS method, the influencing factors were discussed. In addition, the composition of ion-association complex and the reaction mechanism were investigated. The method has a good selectivity. It has been successfully applied for the determination of MBZ in tablets and human urine samples with satisfactory results.3. Study on the interaction between albendazole and erythrosine by absorption, fluorescence and resonance Rayleigh scattering spectra and their applications In Britton-Robinson(BR)(pH=4.0～4.3)buffer solution, albendazole(ABZ) could react with erythrosine(Ery) to form1:1ion-association complex, which not only resulted in the changes of the absorption spectra, the quenching of fluorescence, but also resulted in the great enhancement of RRS. In this work, the spectral characteristics of absorption, fluorescence and RRS, the optimum conditions of the reactions, the influencing factors had been investigated. Thereby, a sensitive, simple, rapid and new method for the determination of ABZ by using Ery as a probe has been developed. The detection limits were2.09ng/mL for RRS method,29.79ng/mL for fluorophotometry and177.47ng/mL for spectrophotometric method. Among them, the sensitive of RRS method was highest. The effects of the reaction of ABZ and Ery on the absorption, fluorescence and resonance Raleigh scattering spectra had been investigated. Meanwhile, the influences of coexisting substances were tested by RRS method, this method can be satisfactorily applied to the determination of ABZ in capsules and human urine samples. The composition and structure of the ion-association complex, the reaction mechanism were discussed.4. Study on the interaction between albendazole and eosin Y by resonance Rayleigh scattering, frequency doubling scattering and fluorescence spectra and their analytical applicationsIn Britton-Robinson(BR)(pH=3.25-3.35)buffer solution, albendazole(ABZ) could react with eosin Y(EY) to form a1:1ion-association complex, which not only results in the quenching of fluorescence, but also resulted in the great enhancement of RRS and FDS. Furthermore, a new RRS spectrum will appear, and the maximum RRS wavelength was located at about356run. The detection limits for ABZ were21.51ng/mL for the fluorophotometry,6.93ng/mL for the RRS method and12.89ng/mL for the FDS method. Among them, the sensitive of RRS method was highest. The experimental conditions were optimized, effects of coexisting substances were evaluated. The methods had been successfully applied to the determination of ABZ in capsules and human urine samples. |
PDF Full Text Request