Organic Fluorescent Probe And Inorganic-Organic Hybrid Chemosensors For Thios

Reactive sulfur species (RSS) includes thiols, S-nitrosothiols, sulfenic acids, and sulfite, and it plays vital roles in regulating intracellular redox status and other fundamental signaling processes involved in human health and disease. So it is very important to carry out selective detection and monitoring for thios. In this thesis, we design and synthesize several probes to investigate their specific properties as fluorescent sensors towards thios.In the first section, we give a review on the background of this work, and brief introduces supramolecular chanemistry, the different mechanisms of fluorescent sensors, the applications of inorganic-organic hybrid materials on fluorescent sensing and fluorescent chemosensors for reactive sulfur species.In the second section, we have designed and synthesized a fluorescent sensors for thios.we conform its structure by using nuclear magnetic resonance(NMR), mass spectrometry and its ultraviolet and fluorescence properties have been characterized. Studies have showed that The sensor can selectively recognize cysteine rather than other natural amino acids in aqueous solution with a rapid response time. Con-focal fluorescence imaging of cells for detecting thiols in vivo was carried out successfully.In the third section, three fluorescent sensors L2, Fe3O4@SiO2-L3and Fe3O4@SiO2-L4were designed and synthesized. The fluorescent response and selectivity to H2S were investigated for Fe3O4@SiO2-L3and Fe3O4@SiO2-L4, respectively. Among them, Fe3O4@SiO2-L3has particular response and selectivity to H2S in acetonitrile solution but the material have a poor solubility in water; and Fe3O4@SiO2-L4has sensitive and selective fluorescent response to H2S in the mixed solvent of acetonitrile and water (4:1).In the fourth section, we mainly described the design and synthesis ofFe304@Si02nanoparticles and studied the influence factors of Fe3O4@SiO2nanoparticles size.