The Preliminary Study Of Absorption And Distribution Of GNPs With Different Hydrophobicity In Mice

Due to its special physicochemical property, nanoparticle is widely used, such as new material, new energy, environmental protection science and medical field. Given this, the chance they are exposured to human is increased rapidly. Nanoparticle can enter into body through oral, breathe, skin and ect. The danger it may bring to us is increased. As reported, kinds of toxicities may be induced, such as pulmonary toxicity, hepatotoxicity, renal toxicity or immunotoxicity.No matter which way the nanoparticle choose to enter into our body, it will be absorpted to blood finally, then distributed to organs along with blood. In the end it is excreted from our body through milk, urine or faeces. This process is ADME of nanoparticle.The toxicity of nanoparticle is closely related to this process. Besides this, the use of nanoparticle in medical fields is related to such process. Then the study of absorption and distribution of nanoparticle is necessity. The absorption and distribution of nanoparticle is directly related to the physicochemical property of nanoparticle. There are many repaorts about the paoperty of nanoparticle, such as size, shape, smoothness, charge and surface modification, all influnce the ADME of nanoparticle. But untill now, there is little reports about how the hydrophobicity of nanoparticle inlunce the absorption and distribution in vivo.In the first part, we used sodium borohydride reduction method to create three different hydrophobic GNPs by adjusting the proportion of two different hydrophobic ligands. These GNPs were characterized by transmission electron microscopy(TEM), dynamic light scattering (DLS) techniqu, Zeta potential and UV-Vis. Ligands were cleaved from GNPs with I2, then quantificated by HPLC-MS. The hydrophobicity of GNPs were characterizated by octanol-water partition coefficient (Log P). The stability of GNPs is tested by DLS and UV-Vis at different pH and time.In the second part, nanoparticle was exposed to mice via oral administration to investigate how different hydrophobicity NPs affect the absorption and distribution in vivo, and use i.v. administration as a contrl to explore the rough bioavialbility after oral administration. In brief, Au content in blood and organs are measured by ICP-MS, Au cotent in blood and in organs reflect its absorption and distribution. We draw conclusion from the second part data:In oral exposure conditions, GNPs in blood is low for all nanoparticles. In most organs, there is no significant difference between different hydrophobicity nanoparticle. But GNPs in small intestine and lung is more for hydrophilic nanoparticle compared to hydrophobic nanoparticle at0.5h and6h, and it is significant. And GNPs is excreted from the body as time goes by. In i.v. conditions, GNP is much more in liver and spleen than other organs.