Researches On The Control Technology Of Monochamus Alternatus By Use Of Entomopathogenic Fungi Carried By Rove Beetle

In this paper,arthropods under the bark of the dead trees caused by pine wilt disease wereinvestigated for looking for the suitable vector insects.Highly virulent strains ofentomopathogenic fungi was screened by biological assay.Control effect of rove beetle carryingthe fungal against Monochamus alternatus was evaluated.The behavior reaction of rove beetle tovolatile matter from its habitat and rove beetle adults were assayed respectively by using“Y”-type olfactometer.The component of volatile matter were analyzed by use of GC-MS for seekingattractant to rove beetle and it will provide theoretical basis for enhancing the search capability ofrove beetle and the control efficiency to wood-boring insects.The main conclusions were asfollows:1. Investigation and analyses of the population structure and ecological niche of arthropodsunder the bark of the dead trees caused by pine wilt disease in the Zijinshan Mountains showedthat temporal-spatial niche width of Staphylinidae was highest,temporal,spatial andtemporal-spatial niche overlap and proportional similarity between Scolytidae,Cerambycidae andStaphylinidae were much higher than that of other arthropods under the bark of the deadtrees,indicating closest relationship among staphylinid and main boring pests.2. Entomopathogenic fungus strain B13showed the highest virulence to Monochamusalternatus.The strain resulted100%mortality to Monochamus alternatus,and the LT50was9d atconcentration of1×107conidia/mL.3. The higher the ratio of rove beetle to Monochamus alternatus,the greater probability ofMonochamus alternatus contact fungal spores,and the higher mortality of infected larvaeMonochamus alternatus.The number of fungal spores carried by rove beetle decreased with thetime extension, more than90%decline was made during the first three days. After7days, Thenumber of fungal spores carried per adult was below104. In the bionic experiment, the total deathrate of larvae Monochamus alternatus infected by the carrying entomopathogenic fungi reached44.68%after60days, while the death rate of longicorn larvae under the barks and shallow xylemreached78.26%. The84.21%accuracy rating of mortality of larvae of monochamus alternatuswas forecasted by invading stains on the Monochamus alternatus of the face.4. The attractant rate of matter A under the bark of the dead trees to female reached90%and to male reached78.89%.While matter B under the bark of the dead trees reached60%and52.22%, respectively.The attractant rate of volatile matter from female rove beetle to male wasrelatively high, which reached93.33%.5. Volatiles compounds from matrix A,matrix B and rove beetle, were extracted by solventextraction method and analyzed by gas chromatography-mass spectrometry. Sixteen, fifteen andeleven compounds were identified in the extracts of matrix A,matrix B and rove beetle, Respectively.Comparing with the results of solid-phase microextraction and gaschromatography-mass spectrometry, the candidate of active components may include dibutylphthalate,9-Nonen-1-ol, hexadecenoic.