| Defensins, as an important member in the innate immune system of animals, have broad-spectrum antimicrobial activity and widely expressed in animal tissues. While, a variety of factors could affect its expression and the probiotic is the one. In our research, Lactobacillus Plantarum was used to affect the expression of defensins in sheep rumen epithelial cells. The stable culture system of rumen epithelial cell was established, and then the rumen filial generation epithelium cells were obtained and identified through morphology, growth curve and immunohistochemical methods. After that, the activated and purified Lactobacillus Plantarum, which were divided into different bacterial concentrations, were cultured with generation rumen epithelial cells in different stimulation time and we measured the expression of SBD-1of the stimulated rumen epithelial cells using qPCR (Real-time quantitative PCR) technology. The results showed that the cultured cells have the typical characteristics of epithelial cells through the identification, and the cells could be used in the following study. After epithelial cells and Lactobacillus Plantarum were co-cultured, we found that the expression of SBD-1has the highest level in the bacterial concentration of109cfu/ml on8H, and there were almost no affections in the bacteria concentration of108,107and106cfu/ml compared with blank control. There was extremely significant difference (P<0.01) of the expression in SBD-1when the cells were stimulated with bacterial concentration of1010and109cfu/ml compared with the concentrations of108,107and106cfu/ml. In our study, the expression of SBD-1could be induced by Lactobacillus plantarum, and the optimum concentration of bacteria was109cfu/ml on8H. |
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