| Soybean, originated in China, is one of the most important grain and oil crops in the world. Development of new cultivars with high and stable yield is always the important target. Annual wild soybean is the ancestral of cultivated soybean. It performs good adaptation, and has abundant genetic variation which can be utilized in soybean breeding. However, there are also some unfavorable traits such as stem prostrate, pod dehiscence. It is necessary to reveal the genetic mechanism of domestication-related traits to understand the evolution trend of these traits and give guidance to soybean breeding. In addition, a molecular genetic linkage map is the base for QTL mapping, map-based gene cloning and marker assisted selection. In the present study,286F2:7lines of a recombinant inbred line population (NJRINP) derived from the cross between Nannong86-4and PI342618B were used to conduct field experiments in2010and2011, and some morphological traits, including100-seed weight, plant height, maturity time, were investigated. Single Sequence Repeat (SSR) and InDel markers were used to construct a soybean genetic linkage map. QTL mapping of the above traits was also carried out. The main results are as follows:1. The parents of NJRTNP were analyzed by using1015pairs of SSR and736pairs of InDel primers. About46.0%and31.3%of the markers performed polymorphic respectively, and201InDel and57SSR markers were selected to investigate the lines. A genetic map with27linkage groups was established by using JionMap3.0software. It contained42SSR markers,183InDel markers and one morphological marker. It had a total distance of1686.1cM, and the average distance was7.5cM between the flanking markers. The length of linkage group varied from7.6cM to115.3cM, and the markers on the linkage group varied from3to16. There were12intervals longer than20cM.2. Plant height, stem diameter, maturity time,100-seed weight in NJRINP population performed normal distribution. There were significant differences among lines for these traits. A total of29QTL for the four traits were detected by the WinQTLCart2.5. Among them, seven QTL for plant height located on chromosome3,11,13,18,19were identified. Three important QTL, qPh13-1in the intervals of Gm13sv293-Gm13sv292on LG-F, qPh18-1in the interval of Gml9sv484-Gml9sv482on LG-G, and qPh19-1in the interval of Gm19sv484-Gm19sv482on LG-L were detected both in2010and2011. Seven QTL for stem diameter were found on chromosome1,2,7,12,18,19,20, and the R2values ranged from1.70%to10.41%. Among the six QTL for maturity time, qMt12-1and qMt10-1were detected in two years. There were eight QTL for100-seed weight, and qSw11-1, qSw02-1, qSw17-1, qSw12-1were also identified in two years.3. QTL/gene tagging for seed coat color, seed bloom, growth habit was conducted. The QTL for seed coat color located on chromosome8was detected in2010and2011. Three loci for seed bloom in the intervals of Gm08sv759-Gm08sv136on LG-A2, Gml5sv336-Gm15sv337on LG-E, and Gm13sv292-Gm13sv613on LG-F-2were found. In two years, two stable QTL to growth habit in the interval of Gm13sv613-Satt334on LG-F and Gm19sv484-Gm19sv482on LG-L was detected by the WinQTLCart2.5. |
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