Effects Of SRTS On The Nitrogen Transformation And Nitrogen-fixing Bacteria Diversity

Salinization Resistance Transgenic Soybean (SRTS) was produced genetic engineering technology,which put the Base aldehyde dehydrogenase gene(BADH) of beet into the cultivated soybean Heinong35. The Yield of SRTS planted in the enclosed area of Daqing-Anda was250-300kg/mu,but the risk and security of soil ecosystem by planting the genetically modified crops to farmland cannot be ignored.As materials of the experiment,five genotypes soybean including SRTS,the parent of SRTS HN-35,HF-50,K and Y-21were compared in the study.The experiment were conducted in this study not only in order to investigate effects of Salinization Resistance Transgenic Soybean (SRTS) on the ammonia-oxidizing bacteria,nitrifying bacteria,ammoniation intensity,nitrification intensity, nitrogen fixation intensity,ammonium nitrogen,nitrate nitrogen,available N and microbial biomass N in rhizospheric soil.but also study effects of Salinization Resistance Transgenic Soybean(SRTS) on the diversity of nitrogen-fixing bacteria in rhizospheric soil by using molecular techniques polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE).From the entire growth period,SRTS soil nitrogen fixing bacteria facilitating role.All varieties of nitrogen fixing bacteria trends SRTS higher than HN-35,Y-21,HF-50and K,SRTS compared with other varieties were increased by10.31%,12.15%,13.31%,16.03%.The SRTS certain extent ammonifiers,to reduce rhizosphere soil ammonifiers quantity.The view from the average of the entire growth period,the rhizosphere soil ammonifiers number HN-35higher than HF-50,SRTS,Y-21and K.HN-35compared with HF-50,SRTS,Y-21and K were increased by3.26%,5.43%,5.81%and7.70%.From the average of the entire growth period,the number of bacteria of rhizosphere soil nitrification was HN-35higher than SRTS,Y-21,HF-50,Kand HN-35,compared with other varieties were increased by1.81%,3.68%,4.65%and10.59%.Analysis of variance,SRTS compared with HN-35at the seedling and flowering no significant differences,in the grain filling stage the SRTS significantly higher than other varieties of soybean, and mature SRTS was significantly suppressed the number of nitrifying bacteria,indicating SRTS rhizosphere soil nitrifying bacteria influence have the stage.From the view of the average of the entire growth period,rhizosphere soil nitrogen fixation strength of HF-50higher than SRTS,Y-21,HN-35and K. the seedling stage and grain filling stage to SRTS rhizosphere soil nitrogen fixation strength with its receptor non-genetically modified soybeans HN-35no significant difference,but the SRTS to be slightly higher than the HN-35flower and pod stage and maturity the SRTS significantly higher than the HN-35.Look from the entire growth period to promote the role the SRTS of rhizosphere soil nitrogen fixation strength. Rhizosphere soil ammoniation intensity was K,HN-35,HF-50,Y-21and SRTS,Compared with Y-21,HF-50,SRTS and K,HN-35were increased by2.07%,22.0%,22.19%and43.02%.This shows that the SRTS to reduce the intensity of rhizosphere soil ammonification. Rhizosphere nitrification intensity from the aggregate level,SRTS higher than Y-21,HF-50,K,HN-35,the SRTS compared with Y-21,HF-50,K and HN-35were increased by12.86%,16.18%,50%and52.9%.The analysis of variance showed a certain extent,SRTS can improve the rhizosphere soil nitrification strength.The aggregate level SRTS can improve soil microbial biomass nitrogen contents,has a certain role in promoting.From the entire growth period,the utilization of ammonium nitrogen is HF-50higher than K,Y-21,HN-35and SRTS.Compared with HF-50,K,Y-21andHN-35,SRTS were reduced by17.68%,13%,12.7%and12.6%.Overall SRTS inhibit their utilization for ammonium nitrogen absorption.The nitrate utilization degree SRTS higher than Y-21,HF-50,K,HN-35and SRTS,the receptor non-genetically modified soybeans HN-35increased compared to5.64%.SRTS played a catalytic role to nitrate.The rhizosphere soil microbial biomass nitrogen SRTS and HN-35difference in growth period,seedling,flower pod SRTS rhizosphere soil microbial biomass nitrogen content of62.5%and310.19%higher than the same period in HN-35content,grain filling stage mature SRTS microbial biomass nitrogen content is insignificant.The SRTS can reduce the rhizosphere soil pH,Instructions the SRTS can improve salinity of saline-alkali soil.DGGE fingerprint analysis,different varieties of soybean exists a certain similarity,some of the bands were common to all.Compared to SRTS,the bands of HN-35no significant difference, SRTS diversity index,with the index and HN-35and HF-50is insignificant,and the similarity of the wild soybean variety resistant line is low.Cluster analysis of five varieties,HF-50and HN-35rhizosphere soil diazotrophs similarity of84%polyethylene into a cluster,SRTS and HF-50, HN-35varieties poly into a cluster,the similarity is78%,the Y-21and the similarity of these three species is69%,K is the lowest similarity is45%.Overall that SRTS slightly affect the diversity of nitrogen-fixing bacteria of the rhizosphere soil,but not significantly.