| The parasitic disease in sheep and goats are one of the diseases that seriously influences the sheep and caprine industry.When sheep and goats influences the parasitic disease, sheep(goats) growing slowly, feed efficiency, meat production and milk production reduced.Some diseases can lead to pregnant sheep(goats) abortion or preterm, may breakdown the survival rate of lambs,even lead to death. Finally lead to an increase in farming costs and decline the economic value.Piroplasmosis is intra-cellular parasitosis,which include Theileriosis and Babesiosis, are parasitized in eryhroeytes or other cells of vertebrata and transmitted by ticks.Can lead to sheep(goats) fever, anemia, weight loss, jaundice, hemoglobinuria and so on, even lead to death,caused huge economic losses to the sheep industry. Seriously restricted the development of the sheep industry.In addition, The introduction of sheep by the non-disease areas on the pear-shaped insects are extremely sensitive to the diseases, the morbidity and mortality are very high.Therefore, The disease is also a serious obstacle to the introduction of improved varieties and local breed improvement work.According to the report, piroplasmosis have happened in many countries and areaes of the world.1043fecal samples were collected from partial regions of China during Jounary2011to Febuary2012. All the samples were stained with some inspection methods. Period of the time, investigate the piroplasmosis in partial regions of Henan province. Molecular characteristics of positive samples from previous study were researched used18S rRNA gene.To understand the prevalence of intestinal parasites infection in goat in China, proviade reference to the prevention and treatment of intestinal parasites in goat.detect1043fecal samples were collected from partial regions of Henan province, Hefei in Anhui province, Yunyang in Chongqing province, Eling in Qinghai province and Hohhot in Inner Mongolia province, there were eleven cities, stained with the Centrifugation method、the Lugol’s iodine-solution staining method and the Sheather’s sugar flotation. Reference to the prevention and treatment of intestinal parasites in goat.Samples of fresh stool obtained from goats, which were detected for nine spieces of Coccidian oocyst, Cryptosporidium oocyst, Giardia cyst, Entamoeba cyst, Strongy-loides eggs Nematodirus egg, Trichuris egg, Moniezia egg and Dicrocoelium dendriticum egg, the total infection rate was95.8%. Many of them were mixed infection, the highest mix infection was six, two spieces or three spieces were rather high, the infection rate of30.3%and31.7%respectively. Coccidian was the ascendant spiece, the infection rate was high to90.8%, infection rate of Dicrocoelium dendriticum egg was0.67%, was the lowest.The infection rate of Giardia were remarkably different in different season, Summer infection rate (11.1%) than in spring (4.72%) and autumn (5.41%). Cryptosporidium infection seen in spring, autumn and winter, The fall infection rate (4.63%) was significantly higher than in spring and winter, The infection rate of Cryptosporidium in lambs (3.77%) was higher than the infection of adult (0.16%), The infection rate of Cryptosporidium in feeding flock (1.58%) slightly higher than the grazing sheep (1.47%). The results show the prevalence of intestinal parasites in goat was very common and serious.Thus, prevention and control of intestinal parasites are needed.In order to understand the species and epidemiological characteristic of Theileria(Babesia) of sheep (goats) in partial region of China. Provide the basis for the development of effective prevention and control measures. During April to December2011, collect212blood samples from Zhengzhou, Zhongmou, Dengfeng, Shangcheng, Lingbao, Lushi, Anyang of Henan Province, Chao yang of Liaoning Province, Linqu of Shandong Province, according to the geographical locations and management systems.Determined samples by microscopic examination of Giemsa-stained blood smears and PCR method (Conventional PCR and nested PCR). Using microscopic examination of blood smears,only can found21positive samples, infection rate was9.90%(21/212), while using PCR method,can found46positive samples, infection rate21.7%(46/212), the result was according with the perious study. Use PCR method detect positive samples by specific primers of Theileria luwenshuni, found that the positive which the study got were all Theileria luwenshuni. But, did not detect Babesia in this investigation. The result indicated that PCR method is more stnsitive and specific than microscopic examination.All the positive samples were Theileria luwenshuni.In order to identify species or genotype of Theileria isolates of sheep(goats) in Henan province, the sequence of46isolates that amplificated based on18S rRNA gene.Then,Blast tool was used to search homological sequences in NCBI, after that, homological sequences were alignmented. Phylogenetic tree and homological analysis were made by some biological softwares such as Clustal X1.81and DNAstar4.0. The results suggest that46isolates that amplificated based on18S rRNA gene of Theileria, the homology with T.luwenshuni (JF719831.1) and Theileria sp. SZH-sheep (HQ844674.1) is100%,and they were all in the same clade by the Phylogenetic analysis. The results show that, all the positive samples were Theileria luwenshuni. |
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