| Foot-and-mouth disease(FMD) is an acute,febrile and highly contagious viral disease which is caused by foot-and-mouth virus disease(FMDV), mainly infected lives cattle, pigs and sheep, and other domestic and wild mammals,,results in severe economic loss for livestock industries and in great national economic loss.FMD has worldwide distribution. Although the whole virus vaccines that inactivated by chemically method play a key role in the control and prevention of FMDV, there are many insufficient in the application parctice of vaccine.Such as the purification of virus particles was difficult and the storage conditions of strains was strict,and the vaccination immune effect was unstable and so on. Most importantly,the potential risk was that the incomplete inactivation of FMDV or the escape of live virus from bio-safety facilities during vaccine producing in a large scale production. In addition, over the years, outbreaks of FMD occur by several FMDV serotypes of O、A and Asisa I alternately occurred in China, single types of vaccine is difficult to effective, leading to vaccine immunization effect is unsatisfactory.In this study,S-layer protein genes were cloned from Lactobacillus acidophilusMG strain genomic DNA. The tertiary structure of SLP of Lactobacillus protein was successfully predicted by CPH model, Swiss-Model, and Swiss-Pdb Viewer3.The slp gene was cloned to the prokaryotic expression vector pGEX-4T-3and constructed the prokaryotic expression vector of pGEX-slp, and finally we obtained the GST fusion protein with the size about70kDa induced by IPTGOn another study, The specific epitope gene of3-type FMDV VP1was chimericed pGEX-slp and expressed in E.coli BL21inducted by IPTG.SDS-PAGE and Western blotting showed that VP1fusion protein was expressed limited and the size was about80kDa.This research laid foundation for further study of multi-type-FMDV-VP1epitope gene embedded subunit gene engineering vaccine job. |
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