| Cynodon dactylon is recognized as one kind of plant having important economic value and insensitiveto tissue culture. It is difficult to obtain and maintain the embryogenic callus, successful reports mostlyfocus on using immature inflorescence as explant, few using caryopsis as explant. With the seeds ofCynodon dactylon （L.） Pers cv. Xinnong No.1as the explants, the effect of hormone, exogenous additiveson embryogenic callus induction, subculture and differentiation is studied, and based on these studies; thestudies on embryogenic maintaining and60Co-γ ray radiation on embryonic callus are carried out. The mainresults of test are as follows:（1） In callus induction, MS medium added with2.00mg/L2,4-D+0.01mg/L6-BA+500mg/L prolineis suitable for callus induction of Cynodon dactylon （L.） Pers cv. Xinnong No.1, and the induction rate canreach71.2%, and the callus form is mostly translucent water-soaked with light yellow particles. Thesubculture medium is MS medium added with2,4-D0.5mg/L+6-BA0.5mg/L+ABA2.0mg/L+GSH2.0mg/L，as is beneficial for embryogenic callus growth; the callus proliferation rate can reach41.3%.Transfering embryogenic callus into1/2MS medium without any hormones for10days, then transferinginto MS medium added with6-BA1.0mg/L, soon the green points become dense, and then differentiateinto seedlings, the callus regeneration frequency is25%, the plantlets rate is16.7%.（2） In embryogenic maintaining trial, MS medium added with2.00mg/L2,4-D+1.00mg/L6-BA+5.0mg/L proline+CuSO42.5mg/L+2.0mg/LGSH is suitable for the embryogenic maintenance, and theembryogenic callus proliferation rate is75.0%, the callus form is yellow dense particles, the plantlets rate is31.3%.（3） In embryonic callus radiation treatment, the best dose is20Gy, and the relatively proliferation rateof embryogenic callus is59.5%, relatively regeneration rate is37.2%. |
