Screening, Expression And The Functional Analysis Of Smu-1Genes From Macrochium Rosenbergii

shrimp culturing is sensitive to temperature of environment. Hightemperature has strong impact on the metabolism of shrimps, water soluble oxygen,water soluble ammonia water, soluble Nitrite nitrogen and the pathogenic bacteria ofenvironment.With the development of aquaculture model intensification and the hotweather of summer, the mortality of shrimp is rising, bringing significant loss toaquaculture industry. Therefore, the study of shrimp thermal stress related mechanismbecome the focus of the current. Using subtractive hybridization library of centralnervous system from Macrochium rosenbergii， smu-1gene was screened whichshowed significant up-regulation under heat stress The amino acids sequences ofsmu-1had high homology with other species, and the protein contained six WDregions at C-terminus of WD40-repeat motif, belonging to WD40-repeat proteinfamily. At the present study, using the real-time PCR technology, it was found that theexpression of smu-1gene up-regulated significantly under heat or cold shock andmental stress(P<0.05, P<0.01) compared with that of control group. But at high andlow osmosis conditions, the smu-1expression had a down-regulatedtendency(P<0.05). Further, smu-1gene was interference by injecting smu-1double-stranded RNA in vivo. The results showed that the survival rate ofMacrochium rosenbergii decreased obviously under heat shock. Our results, to someextent, would provide a theoretical basis for the screening heat-resistantMacrobrachium rosenbergii, and greatly reduce economic losses of shrimp farming.