The Study Of The Community Structures Of Endophytes Within Common Plant Species And Soil Microorganisms In Inner Mongolia Grassland

Microorganisms are very important as decomposers in ecological system.Microrganisms are closely related to plant functions, such as endophytes and soilmicroorganism. conversely, the effects of plant species on the sizes and microbialcommunity structures are important. In order to analysis endophytic and soilmicroorganism community structure of the typical plant in Inner Mongolia grassland anddiscussion the influence of plant-soil feedback effects on endophytic community structure,we take Inner Mongolia Xilin Gol Grassland as research platformand use the TerminalRestriction Fragment Length Polymorphism(T-RFLP) analysis in my paper investigate. Atthe same time, the endophyte fungi which were isolated from grassland plant are cuturedunder laboratory conditions, then classification and identification using molecular biologytechniques. The results of this study are as follows:1. The results of T-RFLP show that endophytic community structure was obviousdifferent between plant species especially endophytic bacterial composition in the plantleaves.we also found that even in the same palnt, the endophytic community structureof different tissue or plant organ has great differences.2. Experimental results for greenhouse cultivation indicate that the plant-soil feedback hasa certain effect for endophytic community structure of different plant. The before-plantspecies will affect the later-plant endophyte.3. The endophyte of Leymus chinensis and Chenopodium glaucum which were cultivatedin greenhouse were isolated and cultured, Through the identification, we found11species belongs to7genus, some harmful and some beneficial. We also found thatthere has similar endophytic composition between leaves and roots, but there are somedifferences even.4. The results showed that different plant species have significant effects on the sizes ofbacteria in both rhizosphere and non-rhizosphere soils and size of fungi in rhizospheresoil. The numbers of bacteria and fungi in the rhizosphere soil were generally higherthan those in non-rhizosphere soils which was particularly significant for fungi. multi-response permutation procedures and principal component analysis of T-RFLPdata showed significant differences of the bacterial and fungal community structurebetween rhizosphere and non-rhizosphere soil for most species, and the fungalcommunity structures of all samples can be divided into the rhizosphere andnon-rhizosphere soils clearly. In addition, the community structure clustered by plantspecies to some extent which is more evident for bacteria.This study reveals that the important influence of plant species, different tissues andplant-soil feedback on endophytic composition, at the same time, explored the effects ofplant species on soil microbial communities in an Inner Mongolia grassland to understandthe ecological consequences of vegetation successions in this region. they all laid afoundation for further study of microrganisms function and riched diversity of InnerMongolia grassland ecosystems.