Analyses With Bioinformatics Method On Leaf Protein Of Thephoto-thermo-sensitive Male Sterile Maize And The Genes In Silico Cloning

By Annexin, a calcium-dependent phosphorylation of proteins, commonly found in is thebody of animals, plants, humans and bacteria, the bacteria has not yet been found.. in the maizemale sterile breeding process, Li Weiping, aobvious type of male sterility characteristics intuitiveshowed no spike flowers albino leaves of this type of corn can be planted in different seasons ofthe period to select and identification of the extract the male sterile line and maintainer linesproteins by SDS-pageelectrophoresis five protein bands of the difference between14.4kD to166kD molecular weight inferred by more than one protein which in the male sterile line withthe mass spectrometry analysis may be of annexinBioinformatics is a subject of research and biological data with the help of computerdatabases and computer algorithms used to process the huge amount of biological data andbiological significance of the information.. annexin protein research corn research is not perfect.This experimentannexin protein amino acid sequences in Genbank search using bioinformaticsmethods to predict a range of information of the annexin protein signal peptide, hydrophobicity,subcellular localization, transmembrane regions, secondary structure. three structures. Use ofbioinformatics to predict the structure and function information, and provide the informationbase for the further study of annexin protein.In silico cloning (In silico cloning) the use of bioinformatics methods for gene cloningmethod is developed with the implementation of the genome project and EST programs.Annexinprotein information probe in silico cloning and cloned the cDNA sequence of the annexinprotein in the maize EST database homology search and filtering. The sequence analysis showedthat the length of1035bp and sequence of the open reading frame and promoter prediction..comparative information analysis with a probe protein structure and function of a novel proteinof the full-length cDNA translation.