Study On Transcriptome Sequencing Of Cotton(Gossypium Hirsutum L.)Resistant Inbred Line Induced By Verticillium Dahliae

Cotton is an important economic crop in China, which plays an important role in satisfying peope’sliving demands and guaranteeing China’s economic development. Cotton Verticillium wilt disease isone of the most harmful diseases in cotton production and is called “Cotton’s cancer”. Study on thechange in transcriptome of cotton Induced by Verticillium dahliae can detect the important genes andbiology pathway related to Verticillium wilt resistance in cotton, and clone the disease resistant-relatedgenes to conduct a genetic improvement for cotton by using genetic engineering technology. In thisstudy, the transcriptome difference between cotton disease resistance inbred induced by Verticilliumdahliae and its control was studied by RNA-seq. Large amount of differential expressed Unigenes andbiology pathway were obtained by sequences assembly and gene annotation. The main results were asfollow:1.By screening total RNA isolation method from cotton young root Suitable for RNA-seq, wefound that the improved guanidine thiocyanate method had the characteristic of high quantity andquality, could satisfy RNA-seq requirements.2.RNA-seq was performed by using Illumina HiSeq:trademark:2000. By De novo assembly anddata analyse,106,445,786Clean Reads and126,402Unigenes were acquired in the two samples, andthe total data size was4.5Gb. After induced by Verticillium dahliae, there were59，856Unigenesshowing up-regulated expression and51,976Unigenes showing down-regulated expression.3.Among all the Unigenes,78.88%(99,712) was annotated into the databases of NR，NT，Swiss-Prot，KEGG，COG，and GO. By gene annotation,93,307Unigenes were annotated into NRdatabase,86,638into NT database,55,955into Swiss-Prot database,49,247into KEGG pathwaydatabase,28,925into GOG database, and73,831into GO database. All the above annotations enrichedthe transcriptome information in cotton.4.Metabolic pathway analysis of differential expression genes showed that there were128pathways, including a lot of metabolic pathways, second metabolic synthesis pathways, plant-pathogeninteraction pathways, and stress-related pathways.5.SSR detection for Unigenes found that the number of mono-, di-, tri-nucleotide repeats was1774,2803, and5378, respectively. The number of total SSR was9728.