Isolation, Identification, And Screening Highly Pathogenic Strains Of Entomopathogenic Fungi To Ixodes Ticks

Hard ticks (Ixodidae) are not only ectoparasites of mammals, but also transmission vectors ofseveral virial, bacterial, spirochetal and protozoan diseases (Most of them are zoonoses). It is the secondvectors of diseases beside mosquitoes. With the public attention shed light on the sanitary andenvironment protection and the increasing resistance of ticks, it has become to a spotlight uisngbiological measures to control hard ticks. Entomopathogenic fungi appear to be one of the mostpromising options for biological control of ticks, due to their wide distribution, low risk to human andanimal health, environmental safety, and high virulence against pests.Therefore,this paper researched the following areas:1:Thirteen isolates of Beauveria bassiana and thirteen isolates of Metarhizium anisopliae wereoriginally obtained from soil and a tick.2: Seven isolates of the fungus B. bassiana were assessed for their lethality against Haemaphysalisqinghaiensis. Fourteen days after exposure to the isolates B.bAT1, B.bAT5and B.bAT7(at108conidiamL-1), the mortality rate had reached100%.3：In this study, we investigated the pathogenicity of thirteen B. bassiana isolates and seven M.anisopliae isolates to the engorged female R.(B.) microplus ticks using different conidial concentrationsof107,108and109conidia mL–1. Fourteen days after treatment, three B. bassiana isolates (B.bAT01,B.bAT03, B.bAT13) and one M. anisopliae isolate (M.aAT04) resulted in100%mortality of engorgedfemale ticks with conidial concentrations of108and109conidia mL–1.