Investigation On Methods Of Determination Health Risk Assessment Of Metals And Antibiotic In Honey Consumed In Zhejiang Province

Honey is a sweet, viscous liquid that bees produce from nectar collected from plant nectaries and store as food.Honey has antioxidant, antibacterial and enhancing immunity and other physiological activities, therefore it is recognized as a food with nutritional properties and a natural product with valuable therapeutic applications.The presence of metals and antibiotic in honey depends on several factors,such as industrial smelter pollution,emissions from factories, by incorrect procedures during the honey processing and conservation phases, and agrochemicals such as cadmium-containing fertilizers, organic mercury, and arsenic-based pesticides used in some countries.As the consumption of honey is booming because of its multiple health-promoting effects, the possible health risks resulting from long-term exposure to metals and antibioticcontained in this honey need to be evaluated. The paper comprises three part, investigation,quantify and health risk assessment of six heavy metals and antibiotic in honey. The results were as follows: Six heavy metals, namelyCu, Zn, Cd, Pb, As and Hg, and three antibiotics,namely sulfanilamide, chloramphenicol,tetracycline,in seven types of unifloral (acacia, linden, citrus, litchi, loquat, jujube and yellow box) and multifloral honeys were determined byFAAS, GF-AAS,HG-AFS,HPLC and LC-MS. Methods were established and the limit of determination, recovery and RSD of the three methods were also studied.HG-AFS method:The limit of determination wasin the range of0.15μg/kg～1.8μg/kg, the recovery was in the range of97.42%～98.94%, the RSD was lessthan1.24%.FAAS/GF-AASmethod:The limit of determination wasin the range of0.8～41μg/kg, the recovery was in the range of98.63%～104.54%, the RSD was in the range of0.752%-1.974%.HPLCmethod:The limit of determination wasin the range of1～10μug/kg, the recovery was in the range of74.0%～96.1%, the RSD was in the range of2.02%～5.62%.LC-MSmethod:The limit of determination was0.08μg/kg, the recovery was in the range of96.9%～104.5%, the RSD was in the range of1.3%～5.1%.All of the four methods can be used to determine.Six heavy metals, namely Cu, Zn, Cd, Pb, As and Hg, and threeantibiotics,namely sulfanilamide,chloramphenicol, tetracycline in eight types of honeys collected from Zhejiang proince were determined.The result indicated that the content of Zn, Pb and Cu in honey was higher, and the average content of Cu was50.38μg/kg,Pb was29.81μg/kg. The average content of chloramphenicol was0.0065μg/kg-0.2106μg/kg.We must attach importance to the problem of chl-oramphenicol.Comparing with safety intake levels for these heavy metals recommended by US Environmental Protection Agency (US EPA) and Joint FAO/WHO Expert Committee on Food Additives (JECFA), the Hazard Quotient (HQ) values for single heavy metal for all six heavy metals and three antibioticswere far below1, indicating no chronic-toxic risks from these heavy metals due to daily consumption of0.01kg of honey for a70kg individual.