Analysis Of Carbamates Pesticide Inbamboo Shoots By High Performance Liquid Chromatography (Hplc)

Bamboo shoots are a nutrient and healthy food, and also the major source for the economy to the certain local government. However, bamboo shoots often suffer from the insect pests, which severely affect the yield and quality of bamboo shoots. The common insect pests of bamboo shoots includes Pantana Phyllostachysae, Bamboo borer, bamboo shoots weevil, bamboo shoots armyworm, and so on. These pests invade the young and fresh parts of bamboo shoots, which is seriously harm to the growth of the bamboo. By now, chemical control is still the major measure to control the disease and insect pests, while the way is also easies to cause the pesticide pollution and residual of edible partly in bamboo shoots, which bring great danger to human health. Carbamate pesticide is the substitute for the highly toxic pesticides, such as organochlorine and organophosphorus pesticides, which is widely used in the pest control on cash crops (grain, vegetable, fruits and tea). Carbamate pesticide could inhibit the acetylcholinesterase in human to cause acute toxicity, or even threat the human life. Analysis methods for Carbamate pesticide include chromatography, gas chromatography-mass spectrometry (GC-MS), liquid chromatogram-mass spectrometry (LC-MS) and enzyme inhibition assay. LC-MS method mentioned above is well used, however, the cost of which is too high that general laboratory couldn’t afford. Our study is aimed to explore a simple, efficient and fast analytical technique by high performance liquid chromatography (HPLC) to detect the content of Carbamate pesticide residual on bamboo shoots.In this study, a fast and efficient analysis method to detect Carbamate pesticide residual on bamboo shoots was built through HPLC. The enrichment and separation of Carbofuran and Carbaryl in bamboo shoots could be achieved by FLORISIL SPE column using acetonitrile as extraction solvent and ethyl acetate as the sample solvent. The effective detection of Carbofuran and Carbaryl was achieved using methanol-water system as the mobile phase through gradient elution at25℃. Two level of Carbofuran,0.1mg/kg and0.5mg/kg, were added to the blank bamboo shoot samples, and then the content of Carbofuran was detected according to the method mentioned above, the average recovery of which were84.9%and89.5%, the relative deviation (RSD) of which were7.26%and5.92%and the minimum detection limit(MDL) of which was0.010mg/kg. Two level of Carbaryl,0.1mg/kg and0.5mg/kg, were added to the blank bamboo shoot samples, and then the content of Carbaryl was detected according to the method mentioned above, the average recovery of which were82.3%and89.8%, the relative deviation (RSD) of which were7.63%and6.12%and theMDL of which was0.008mg/kg. Results indicat that the recovery and precision are in line with the requirements for analyse, which means the method is suitable for the detection of Carbofuran and Carbaryl residual in bamboo shoots.