The Epidemiological Investigation Of Haemophilus Parasuis About Shihezi Reclamation

Haemophilus parasuis(Haemophilus parasuis, Hps) disease Haemophilus parasuis disease swine Glasser, cause swine systemic cellulose polyserositis, arthritis, pleurisy and meningitis. Caused huge economic losses to the swine industry.In this study, the the Shihezi scale swine farms Vice swine Haemophilus disease conducted a systematic investigation and study, serological survey, molecular epidemiology, Pathogen identification and susceptibility testing and other aspects of Shihezi ReclamationHaemophilus parasuis prevalent status of the system, and provide a scientific basis for the Reclamation Haemophilus parasuis disease prevention and control.1.Understanding of swine disease Haemophilus in Shihezi infection, using serological methods Haemophilus parasuis antibody detection, and other respiratory diseases mixed infection serological survey of antibody positive sera. Results:The the HPS330serum samples were antibody positive rate was60.3%(199/330),12farms infected with the positive rate was91.67%(11/12);199positive sera,24.62%(49/199) infection H1N1subtypes SIV,49.25%(98/199) infection of the MHP,84.42%(168/199) infected with PCV2,35.18%(70/199) infected with the APP,49.75%(99/199) infected with SS2. Description Reclamation most of the scale farms to HPS infection exists and mixed infection is very serious.2.Collected in Shihezi12large-scale farms has obvious symptoms of respiratory disease of swines lung tissue, pleural effusion, pericardial fluid, such as diseased of105copies, Isolation, culture, biochemical identification, PCR detection and sequencederived analysis and comparison. Results:isolated from105copies disease compound Haemophilus parasuis4,29, PCR testing positive detection rate of27.62%(29/105); sequencing results in NCBI using BLAST, which SHZ0001, strains of PCR amplification products were sequenced and the results of the Gene Bank Haemophilus parasuis AB004030.1, DQ229053.1, FJ667951.1homology of99%and SHZ0002strain the Gene Bank Vice swine Haemophilus GU193456.1the homology reaches FJ667951.1, CP001321.1100%.3. To filter out on the the Shihezi farms of swine Haemophilus disease therapy to reduce the economic losses of the farms, select the commonly used antibiotics:ciprofloxacin, kanamycin, oxytetracycline, enrofloxacin, Qinglarge neomycin and other11kinds of antibiotics susceptibility testing. The results showed that:isolates highly sensitive to ceftiofur sodium, florfenicol, ciprofloxacin, oxytetracycline, enrofloxacin, azithromycin, sulfadiazine, penicillin medium sensitive to kanamycin, streptomycin is not sensitive to strong resistance. Conclusion:Shihezi Reclamation scale swine farms in Haemophilus parasuis has varying degrees of resistance, clinical treatment on some antibiotics can choose ceftiofur sodium, florfenicol, ciprofloxacin highly sensitive drugs to carry out the treatment.