Study Of Soil Microorganism Along Elevational Gradients On Typical Aiphine Meadow In Sanjiangyuan Nature Reserve

Soil microorganism is the most sensitive monoid to environment change inecological system, the study for soil microbial diversity and its influence factors cancontribute to evalute the response of the global environmental change to soilmicrobial diversity, also make for the terrestrial ecosystem management and functionregulation. In the paper, taking the typical alpine meadow in the Sanjiangyuan NatureReserve for object of study, there are65quadrats in5sample plots along altitudinalgradient， I have studied the diversity of soil microorganism and analyzed itsdistribution patter of functional gene and impact factors, the results as fllows:(1) The5sample plots in alpine meadow have21families,51genuses and74kindsof plant. The Kobresia schoenoides, Kobresia pygmaea, Kobresia humilis and othersuch korbresia plants take the ascendant part in the altitudinal gradient from4141mto4788m; Stipacapillata, Poaannua L., Elymus nutans and other grass family plantstake the most part in the altitudinal gradient between3221m and3490m.（2）Soil moisture decreased within creasing elevation and soil temperatureincreased instead. The SOC、TN、TP、TS in alpine kobresia meadow exceed thosein the washigherthan and alpine weeds meadow, but the patassium content isopposite.Influence of elevational gradients to alpine meadow communities isrelatively obvious. Soil moisture and temperature change with different elevation,thus it can affect the composition and distribution of plant communities, and theother environmental factors are pH, soil temperature, soil moisture, TN, TP, TK andTS.etc.(3)The GeoChip4.0with functional genes of C, N, P and S cycle has tested80413functional genes, model correspondence analysis shows that the main soil factors toimpact the microflora composition are pH, soil temperature, soil moisture, TN, TPand rapid available phosphorus, main plant factors are plant species richness.(4)Diversity indexes of functional genes of C, N, P and S cycle in soil microbialcommunity basically show no differences, indexes of Kobresia schoenoides-Kobresia pygmaea are the lowest, Kobresia schoenoides are the higest; gene signal strength variation trend of the N, P and S cycle almostly keep the same, the orderfrom big to small is: Kobresia schoenoides> Stipacapillata–Poaannua L．>Kobresia schoenoides–Kobresia pygmaea>Korbresia>Elymus natans.(5)In the C cycling genes of soil microbial community, the diversity indexes ofcarbon fixtion gene,C degradation gene and mathane cycling genes exhibt the orderfrom big to small is: Stipacapillata–Poaannua L.>Elymus natans.>Kobresiaschoenoides> Korbresia>Kobresia schoenoides–Kobresia pygmaea. The three genesignal strength variation trend almostly keep the same with the C cycling genestrend, the diminishing order is:Kobresia schoenoides>Stipacapillata–PoaannuaL.> Kobresia schoenoides–Kobresia pygmaea>Korbresia>Elymus natans. Themain soil factors to impact the microflora composition of C cycle are pH,temperature, moisture, TN, TP, SOC, LOC, plant species richness and above-groundbiomass etc.