| Quinolones (QNs) is a new synthesis antibiotics, which can restain synthesis of bacteria’s DNA. However with these drugs were abused, these generate tolerance and toxic and side-effect have make many coutries’s attention.In order to protect the health of consumers, The research of detecting technique for QNs residue in food is very important. Rapid colloidal gold immunochromatography test strip established is sensitive and that the procedure of determination is simple and quick, It was proved to be widely used to detect the quinolones residues in milk.Conjugates of three haptens NOR with Bovine serum albumin (BSA) is used to immunize rabbits, and the broad specificity polyclonal antibodies were produced. This paper detection specificity and sensitivity of polyclonal antibodies, the results showed that the anti-NOR have a broad-specificity, which can detect16QNs. Then a competitive assay based on the principle of immunoassay using colloidal gold as tracer for the rapid on-site detection of quinolones residues in food samples was studied. Gold chloride was reduced with sodium citrate to make20nm colloidal gold, using the anti-NOR antibody and NOR-OVA, the colloidal gold immunochromatographic rapid test strip was developed for the determination of quinolones residues. The limit of detection of16quinolones is1ng/mL for lOng/mL.Milk samples were used as real sample (determined to be free of quinolones by HPLC) for analysis, These food samples were spiked with quinolones. Rate of recovery is preferably. The LOD of in milk samples were equal to or lower than maximum residue limit. The results obtained by test strip were coherence with the results obtained by ELISA and test strip could be reserved for more than2months at4℃.The method established is sensitive, rapid and simple, and the results could be obtained within10minutes. All the results proved that the two test strips could be widely used to detect the quinolones residues in animal foods on-site. |
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