Biodiversity And Genetic Structure Of Southern China Population Of Beauveria Bassiana

Beauveria bassiana as a kind of important pathogenic fungi insects, because of itshost range widely, facilitate quantity production remarkable characteristic, have beenwidely developed for microbiological pesticide in the pest to widely used. For in this, andin view of China’s southern Beauveria bassiana spore population differentiation andgenetic structure of the broad and deeply study, so as to achieve better reveal Beauveriabassiana genetic regularity and change of purpose, are particularly important. This isbecause that way, we can use the pest born genetic law of fungi to guide microbial controland even beneficial insects pests of the protection of natural resources use to work, can inrich germplasm resources in our country at the same time, better maintain good forestecological system stability and continuity. This paper present the more mature ISSR(simple repeat sequence interval) molecular marker technology to our country southBeauveria bassiana genetic diversity index and the genetic structure of the population, andmolecular ecology theory with organic unifies in together, according to geographicprovince and discussed according to the division of the classification of the source hostpopulation or the population gene flow between strength, genetic differentiation coefficientfactors in the ball spore beauveria bassiana strains group of genetic differentiation ofinfluence.1. For570strains isolated screening of good stability eight Beauveria bassiana primer,genetic polymorphisms index analysis, the results show that the eight primer afterISSR-PCR amplification altogether by163clear amplification sites, a single primeramplification of sites ranging from17to article26, all of the primer polymorphic locipercentage (PPL) high, numerical reached100%, and can be a better repeatability, each ofthe primer Is、Heand were higher: Hefrom0.216to0.368, with an average of0.293; Isfrom0.371to0.550, with an average of0.464. Molecular markers that ISSR is to evaluateBeauveria bassiana strains group of genetic diversity of effective methods.2. For the south570strains isolated the genetic diversity of the analysis results showthat: in the south of Beauveria bassiana polymorphic loci large population percentage(PPL) is100%, and the two of them respectively genetic diversity index for Nei geneticdiversity index (He) is0.293, Shannon information index (Is) to0.464. The population ofthe diversity index between level differences, various provinces and population PPL arehigher, which Is the lowest Zhejiang and population, the PPL numerical is69.33%, and thehighest population and hunan province and the population, the index was100%betweenPPL, Herange in0.172(Zhejiang) to0.279(Anhui) between, Isin the range of0.281 (Zhejiang) to0.449(Anhui) between, and in the two indexes of the sensitivity of the Ishigher; Different host mesh division of the population PPL range between26.99%~100%,among them: Lepidoptera、Coleoptera、Diptera、Hymenoptera、Homoptera purpose PPLindex are higher, to100%, on the one hand, that the above five orders of the hostpopulation genetic diversity and generally high, on the other hand also out and some hostmesh sample number less relevant. HeZhongGe wing mesh minimum range, Is0.135, thehighest and lepidoptera,0.297, Isin the range of0.187(leather wing mesh) to0.467(lepidoptera) between, visible and the two HeIsthe size of the index correlation moreapparent. The above two diversity index all show: in southern China Beauveria bassianagenetic diversity level is higher, the heterogeneity of the population is stronger. Amongthem the genetic diversity of population of Anhui province and population highestheterogeneity, Zhejiang and the genetic diversity of population and the populationheterogeneity minimum; Lepidoptera separation of genetic diversity and plant populationhighest heterogeneity, leather wing mesh separation strains of the genetic diversity andminimum population heterogeneity.3. According to geographic province for classification of nine and population geneticdifferentiation between the results show that: nine different provinces and the totalpopulation genetic diversity degrees Htan average of0.291, the population of genes withinthe various degrees an average of0.237Hs, genetic differentiation coefficient Gstanaverage of0.187, with an average of1.088Nm gene flow;10different host mesh and theHtaverage of0.292population, Hs0.258average, Gstaveraged0.118, an average of1.866Nm. In this big population exist a high level of genetic differentiation, it is worth notingthat the variation of the populations in significantly higher than the population of thevariation between, it may be due to the population genetic communication between thecause. Popgene1.32software to use respectively nine provinces and population and10host mesh and calculated the population between two two gene flow and geneticdifferentiation coefficient, the results showed that: Guangdong and Yunnan strains betweenDermaptera and Araneida separation between plant genetic differentiation coefficient is thelargest, Anhui and Hunan and between population and coleoptera and membraneseparation between plant wings orders minimum; Each host mesh and population geneticdifferentiation between small, except Dermaptera and Araneida, Beauveria bassiana indifferent orders between host and no strong designed the sex.4. According to the classification and geographical provinces according to host of theclassification of the population between two and two genetic consistent degrees and genetic distance analysis, this paper concludes that the use of Popgene1.32respectivelysoftware to9provinces and population and10host mesh and the population between twotwo genetic consistent degrees and genetic distance is analyzed, and the results show thatprovinces: I from0.825(Guangdong/Yunnan) to0.982(Anhui/Hunan), D from0.018(Anhui/Hunan) to0.192(Guangdong/Yunnan), Guizhou, Yunnan, Zhejiang, Anhui, Jiangxi,Fujian, Hunan and with most of the population are between I bigger; And Guangxi,Guangdong with all the population is smaller between; Host results show that: I in0.868(Dermaptera/Araneida) to0.995(coleoptera/Hymenoptera), and between the D in0.005(coleoptera/Hymenoptera) to0.142(Dermaptera/Araneida) between. Among them,Guangdong and Yunnan strains between Dermaptera and Araneida separation plant geneticdistance between the largest, Anhui and Hunan and between population and coleoptera andHymenoptera separation between plant wings orders the smallest. According to the host ofthe classification of the eyes and the average between population genetic differentiationcoefficient and average genetic distance according to the provinces were lower than that ofthe division of the population of the relevant index.5. With MEGA4.0UPGMA method of the generation of software based on thegenetic distance of the population tree clustering figure analysis: south of Beauveriabassiana in geography, host mesh and Anhui provinces are three list clustering analysis,according to the provinces of nine and population division between the clustering figureshows that some work far apart in provinces subspecies cluster together, and individualadjacent provinces population and not the together, so the genetic lineage and geographicdistance without obvious relationship between; According to the host mesh of theclassification of the population of between10and clustering figure shows that:10differentstrains of the host objective separation population, in addition to their wings purpose ofseparation plant into a and far away from the outside, the others each separation plantgeneral purpose together into one. The system with the10orders tree in the evolutionarysystem outline insects differ greatly. The outline of spider purpose insects separationstrains of separation plant wings purpose than with other8orders the separation of insectsstrains of the genetic relationship more close, so the genetic lineage and host source alsohave no obvious between the relevant relations; In FigTree software based on southernAnhui province with Beauveria bassiana and population of412strains of the geneticdistance between two of the generation of clustering figure, three collecting zone therelationship between the strains the show for random state, and not with the differentcollecting zone and any gathered trend, thus with small area by412strains of a large sample of Beauveria bassiana further indicates that the Beauveria bassiana genetic lineageand its geographical origin not related.6. Use DPS v7.05software, to570strains between different genetic based onindividual do the distance UPGMA cluster analysis method. According to each individualgenetic distance between strains of the generation of clustering results show that, besidesthe minority population structure and population has obvious regional characteristicsoutside, most of the population still is random pedigree analysis. Among them:(1)Guangdong and Guangxi’s most strains together form of south China.(2) The otherprovinces and population each randomly crisscross form I and II two hybrid is; This isobviously because of Guizhou, Yunnan, Zhejiang, Anhui, Jiangxi, Fujian and Hunanpopulation with most and the genetic distance between are minor cause.(3) Thedevelopment in the mixed in Anhui province and other all of the population of each branch;But Yunnan branch in the hybrid mix of all the other than I of the population in thebranches.To sum up, although our country south of Beauveria bassiana genetic differentiationshow some of the regional features, but is the result of the selection pressure differentiation,mainly by each province is different collecting zone and collecting zone internal differentbetween micro habitat of the difference. Come to the following conclusion: Beauveriabassiana genetic lineage and geographic distance and host variation not related.