The Preliminary Research Of Tissue Culture About Ulmus Pumila Linn.in Vitro Regeneration System

In this study, seeds and stems of Ulmus pumila Linn. as the initial material was studied, through initial cultureis, subculture, rooting access to stable sterile seedlings,and the seedling were hardened、transplanted.Through leaves、cotyledons、stems on the sterile seedling and it is from outdoor induce and differentiate callus. Then adventitious buds develop into whole plants,we can establish in vitro regeneration system of Ulmus pumila. Elm genetically engineered for the future provide theoretical basis for improved crops and factory nursery as base material. The main results were as follows:1. Starting cultureIn the seed germination study, the combination of sterile water and cotton wool is sprout fastest of all; The derived stem In mid-June is the highest induced rate.The rate of obtained plantlets was76.8%,the rate of browning is17.6%.2. Primary cultureThe most suitable culture media for the seeds differentiation is MS+6-BA0.1mg·l-1+IBA0.025mg·l-1.The best medium for buds growing is MS+6-BA0.1mg·l-1+IBA0.005mg·l-1.The differentiation rate of plantlets is79.3%,the average height is2.75cm,the average number of leaves per seedling is4.7.3. Subculture stageThe best subculture cycle is25-30d, the best subculture medium is MS+6-BA0.1mg·l-1+IBA0.01mg·l-1,the average height is3.2cm,Multiplication factor is4.2.4. The experiments of Ulmus pumila Linn, rooting and transplanting shows that the bestrooting medium is1/2MS+IBA0.05mg·l-1,the average length of root per is2.1cm.5. Callus cultureThe best sampling time of leaves outdoor is the late April,the induced rate is82.7%.The best callus induction media:MS+TDZ0.1mg·l-1+IBA0.2mg·l-1,the induced rate is89.2%.Cotyledon callus induction media:MS+TDZ0.05mg·l-1+IBA0.1mg·l-1,the induced rate is89.7%.The most appropriate medium for the differentiation of cotyledons and leaves is MS+TDZ0.2mg·l-1+IBA0.05mg·l-1.It shows the high budding,the number is3.8and the differentiation rate is87.6%.We also select stem segments of Ulmus pumila induce into callus in the study.The highest rate of the induction medium is MS+NAA0.5mg·l-1+6-BA0.2mg·l-1.The average number of induced bud is4.2,the differentiation rate is89.0%.In the process of callus induction,the induction rate of stem segment is the highest,the speed of cotyledon forming callus is the fastest.