Analysis Of Cellulase And Protease And Activity Of Goose Intestine And Screen On Bacteria Capable Of Degrading

In order to explore the activity of cellulase and protease of geese intestine, and separated from the geese intestine to filter out the efficient cellulose degradation strains, this paper mesure the cellulase activity and protease activity of Yangjiang geese and Landes geese to compare the size of the activity of these enzymes in different bowel; Separated out two efficient degradation of cellulose strains from Yangjiang geese and study the best conditions of enzyme production. Finally, make preliminary identification with the characteristics of morphological and molecular biology to look forward to provide a scientific basis for the reasonable use of green fodder of geese.Test one:the mesurement of the cellulase activity and protease activity of Yangjiang geese and Landes geese.The results shuowed that the descending order of cellulase activity of different bowels is:The cellulose activities of every intestinal in Landes Goose is jejunum> duodenum> ileum> cecum> gizzard; The cellulose activities of every intestinal in Yangjiang goose is duodenum> jejunum> ileum> gizzard> cecum; the descending order of protease activity of different bowels is:The protease activities of every intestinal in Landes Goose is jejunum>duodenum> cecum> ileum. The protease activities of every intestinal in Yangjiang Goose is duodenum> jejunum> ileum> cecumTest two:Isolated and screened the cellulose degradation strains from Yangjiang geese bowels and then study the enzyme production strains under different temperature, time, pH value conditions, so as to optimize the conditions of enzyme production.The results shuowed that it gets19degrading bacteria which can break down cellulose and gets2strains with high degradation ability. It is the duodenum D-1and the ileal J-1. After conditions optimization, the strain maintain pH=6at conditions of42℃, and reach the highest enzyme activity of0.89U/mL after48hours. Although the two strains of the best fermentation conditions are similar, the strain J-1has a higher enzyme activity compared to the D-1, so take the strain J-1as the next step strain identification test sample.Test three:identify the morphological and molecular biology of purpose strains by means of16S rDNA technology.Bacterial16SrDNA sequence comparison with the Gene Bank inside the database, the results show that the similarity of sequence of J-1and Bacillus subtilis is99%. Also combined with the dentified tresults of morphology, Gram stain and molecular biology, initially determined that the J-1strain is the bacillus subtilis of the genus Bacillus.