The Study Of Soil Microbial Diversity In Kumtag Desert

To definitude microbial diversity of Kumtag desert, we conducted a survey about Kumtagmicrobial resources, collection, identification and preservation of precious microbial resourcesto provide an important foundation materials for extremophiles protection, development andutilization of microbial resources and the excavations of important genes. This paper obtainedthe following main results:The diluted coating method was used to diluted coating the99Kumtag soil samples onR2A and NA solid medium. Compared with other desert, Kumtag soil samples’ microbialbiomass as a whole is small, and most samples’ microbial biomass orders of magnitude lessthan103. Which come from vegetation quadrat samples’ microbial biomass is higher than thesamples that without vegetation quadrat.The400strains’16S rDNA sequencing results showed that, they belong to the Bacillus,Brevibacterium, Pseudomonas, Acinetobacter, Alcaligenes, Paenibacillus, Arthrobacter,Brevibacillus, Streptomyce and other15kinds of genus. The Bacillus accounted for78%, it isthe superiority genus of microbial community in Kumtag. There are35potential new strainsthat compared with the published strains’ sequence similarity less than97%, accounting for8.75%of the total number of strains. Classified statistics showed that: there are three speciesbelong to Bacillus, three species are Streptomyces, the rest are Arthrobacter, Rhodococcus,Nocardia and Staphylococcus one each, that amount to6genera and10species.Selecting two representative quadrats to contrast, the results showed that bacterial speciesof the quadrat contained with different plant community composition, the same strains anddifferent strains of two typical representative quadrats ratio of1:1.