The Analysis Of The Heredity Character Variance Of The Progeny （of Jinda78） Irradiated By⁶⁰Co-ray

In this study, we got two years phenotypes and genotypes. Assocation analysis using the offspring population of M4and M5of Jinda78, irradiated by60Co γ-ray is to detect correlations between genotypes and phenotypes in a sample of individuals.This study analyzed19phenotypic characters(plant weight, plant height, pod height, stem diameter, effective branch, the number of nodes on main stem, the number of pods on main stem, pods per branch,1-seed pod number,2-seed pod number,3-seed pod number,4-seed pod number, shriveled pods, pest-bitten seed number, total pods,50-seed weight, weight per plant, protein content and fat content) and18simple-sequence repeat(SSR) loci. I used correlation analysis, principal component analysis and cluster analysis method. The detailed results were obtained as follows:1. Through the observation of the morphology, the separation and variation of the leaf shape, flower color, hilum color, breeding period and hair color were found in different degrees in the offspring.2. The correlation and principal component analysis of phenotypic traits about the parental and mutagenic offspring groups showed that some traits were significantly correlated with yield per plant. And through the cluster of the principal component values, the population was divided into three groups.3. The polymorphism information contents (PICs) of the SSR loci ranged from0.029-0.860, Shannon’s index ranged from0.078-2.082, genetic similarity coefficients ranged from0.625-1.000. The analysis of molecular markers also indicated abundant genetic variation could result from the y-ray radiation. This provided detective method and theoretic base for germplasm enhancement by using y-ray radiation.4. In this study, phenotypes and genotypes were used to analyze the genetic diversity of soybean radiation mutagenesis generations material, The study at two aspects of morphology and molecular markers reveals that radiation breeding could create abundant genetic variation and reflect genetic diversity of mutation progeny materials more fully. The results provided a genetic basis and techniques for improving germplasm quality by molecular breeding in soybean.