| Study was conducted in Tianjin Beichen dairy farm with11postive,18negative transgenic humanalpha-lactalbumin calves and15non-transgenic calves to investigate the growth regular pattern oftransgenic cows,11turn alpha-lactalbumin transgenic cattle and18transgenic negative cattle were theexperimental group,15the traditional Holstein cows were the control group. Designed three tests, growthregular pattern of cows which turned human alpha-lactalbumin gene, nutrient digestibility of the carryinghuman alpha-lactalbumin gene cattle and physiological and biochemical indexes study of turn alpha-wheyprotein gene cattle, the analysis of growth between transgenic animals and traditional animals.Test: Growth regular pattern of turn human alpha-lactalbumin gene bovine, calves were selected to feedthree months, divided into two parts that contained before weaning (birth to2months of age) and weaning(3months), calves were gived6liters of milk everyday in before weaning,3times feeding everyday andevery two liters, pellet feed and water were fed ad libitum. Before weaning,weight, bust, body height, bodylength, the circumference and body temperature of three kinds of cattle were measured. The same time,normal cattle, positive cattle and negative cattle daily gain were0.67,0.68,0.69kg, analysis of variancefound that the difference was not significant (P>0.05). After weaning, the cows were divided into threefeeding group, daily gain of three groups were300,600,900g, pellet feed provided the necessary energy,alfalfa and water ad libitum,tested weight, bust, body height, body length, the circumference. Afterweaning，daily gain of300g group of non-transgenic cattle, transgenic cattle and negative cattle whichdaily gain were0.92,0.94,0.89kg, the difference was not significant（P>0.05）,. Daily gain of600g and900g group of non-genetically modified transgenic positive and negative cattle weight gain was also nosignificant difference. Three feeding levels, body height, chest and other indicators of non-transgenic cattle,transgenic cattle and negative cattle which P values are greater than0.05, the difference was notsignificant..Test: Nutrient digestibility of turn human alpha-lactalbumin gene cattle, calves were selected to feedthree months, divided into two parts that contained before weaning (birth to2months of age) and weaning(3months), calves were gived6liters of milk everyday in before weaning,3times feeding everyday andevery two liters, pellet feed and water were fed ad libitum. Fully closing the cows’ dung for3days,analysed the digestibility of DM, CP, NDF and ADF, before weaning cows’ dry matter digestibility were85%,87%and83%, the difference was not significant（P>0.05）. After weaning, the cows were dividedinto three feeding group, daily gain of three groups were300,600,900g, pellet feed provided thenecessary energy, alfalfa and water ad libitum, analysed the digestibility of DM, CP, NDF and ADF. Daily gain300g group, dry matter digestibility of non-transgenic cattle, transgenic cattle and transgenic negativecattle were71%,62%and65%,the difference was not significant（P>0.05）; daily gain600g group, drymatter digestibility of non-transgenic cattle, transgenic positive cattle and transgenic negative cattle were71%,73%and67%,the difference was not significant（P>0.05）; daily gain900g group,dry matterdigestibility of non-transgenic cattle, transgenic cattle and transgenic negative cattle were73%,68%and71%, respectively, the difference was not significant（P>0.05）, the difference was not significant.Dailygain300g group, CP digestibility of non-transgenic cattle, transgenic cattle and transgenic negative cattlewere75%,71%and73%,the difference was not significant（P>0.05）; daily gain600g group, CPdigestibility of non-transgenic cattle, transgenic positive cattle and transgenic negative cattle were74%,79%and72%,the difference was not significant（P>0.05）; daily gain900g group, CP digestibility ofnon-transgenic cattle, transgenic cattle and transgenic negative cattle were75%,73%and75%, to do thedifference was not significan（tP>0.05）. Daily gain300g group, NDF digestibility of non-transgenic cattle,transgenic cattle and transgenic negative cattle were55%,44%and44%,the difference was not significant（P>0.05）; daily gain600g group, NDF digestibility of non-transgenic cattle, transgenic positive cattleand transgenic negative cattle were40%,45%and42%，the difference was not significan（tP>0.05）; dailygain900g group, NDF digestibility of non-transgenic cattle, transgenic cattle and transgenic negative cattlewere56%,34%and48%, the difference was significant（P<0.05）, for multiple comparisons, there weresignificant differences between transgenic positive cattle and non-transgenic cattle, namely P=0.003.thesame, there were significant differences between transgenic positive cattle and transgenic negative cattle,namely P=0.043. Daily gain300g group, ADF digestibility of non-transgenic cattle, transgenic cattle andtransgenic negative cattle were49%,36%and35%,the difference was not significant（P>0.05）; dailygain600g group, ADF digestibility of non-transgenic cattle, transgenic positive cattle and transgenicnegative cattle were40%,34%and39%,the difference was not significant（P>0.05）; daily gain900ggroup, ADF digestibility of non-transgenic cattle, transgenic cattle and transgenic negative cattle were55%,28%and50%, the difference was significant（P>0.05）, for multiple comparisons, there were significantdifferences between transgenic positive cattle and non-transgenic cattle, namely P=0.002.the same, therewere significant differences between transgenic positive cattle and transgenic negative cattle, namely P=0.007.Test: Physiological and biochemical indexes of turn alpha-lactalbumin gene cattle, calves were selectedto feed three months, divided into two parts that contained before weaning (birth to2months of age) andweaning (3months), calves were gived6liters of milk everyday in before weaning,3times feedingeveryday and every two liters, pellet feed and water were fed ad libitum. Collecded blood of calves beforefeeding colostrum, after about4hours collecded blood (before second feeding) again, then at10,28,52,100,148hour, blood samples was collected, every10days collecded blood. IgG, GH, T4, Ins, Ach and T of bovine serum was analysed, got the IgG were19.7,19.1and19.5μg,/mL of normalbovine,transgenic cattle and transgenic negative cattle before weaning, the difference was not significant（P>0.05）. After weaning, the cows were divided into three feeding group, daily gain of three groupswere300,600,900g, pellet feed provided the necessary energy, alfalfa and water ad libitum, analysed IgG,GH, T4, Ins, Ach and T. Daily gain300g group,90-day-old IgG of non-transgenic cattle, transgenic cattle,transgenic negative cattle were25.8,24.64and22.43μg/mL, the difference was not significant（P>0.05）;daily gain600g group,90-day-old IgG of non-transgenic cattle, transgenic cattle, transgenic negative cattlewere19.67,19.95and22.28μg/mL, the difference was not significant（P>0.05）; daily gain900g group,90-day-old IgG of non-transgenic cattle, transgenic cattle, transgenic negative cattle were22.41,25.27and20.6μg/mL, the difference was not significant（P>0.05）. Three feeding levels, GH, T4, Ins and otherindicators of non-transgenic cattle, transgenic cattle and negative cattle which P values are greater than0.05, the difference was not significant.. |
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