Effects Of Cigarette Smoke On Mice Flora In Vivo And In Vitro And Diabetic Rats

Cigarette smoke is a mixture of extremely complex composed of thousands of chemicals, many ofwhich components have certain toxic effects on cells or organism. In order to study the harmful effect ofcigarette smoke on the health of the body, the related applied basic research was carried out in the presenteddissertation mainly from the aspects of toxic effect of cigarette smoke on microflora in vitro and in vivoand diabetes.First, the change in the number of colonies from the model organism of intestinal and oral flora(Bifidobacteria, Lactobacilli, Escherichia coli, Streptococcus mutans) in mice was compared and analyzedby using gavage treatment of smoke condensate and passive smoking treatment;Second, combining the above experiment in vivo, in order to explore the mechanism, then we developeach of these bacteria in vitro, added different doses of cigarette smoke extract in the medium, eatimatedthe effect of cigarette smoke extract on the quantity of extracellular polysaccharide(EPS), intracellularpolysaccharide(IPS) and lactic acid.Third, the effect different doses of cigarette smoke extract on blood glucose, blood lipid and relatedindicators of experimental diabetic rats induced by streptozotocin(STZ) was preliminary investigated, andthe hypoglycemic effect of Stropharia Rugoso-annulata was initially explored for the prevention andcontrol of diabetes.The results show that:(1) the gavage treatment of smoke condensate in mice make the number of colonies in intestinalprobiotics (Lactobacillus and Bifidobacterium) was significantly reduced(P <0.05), no significantdifference between the number of colonies in intestinal pathogenic bacteria (Escherichia coli)(P>0.05),the number of colony in oral cariogenic bacteria (Streptococcus mutans, S.mutans) was significantlyincreased (P <0.05), the treatment of passive smoking can make the number of colonies in S.mutans weresignificantly increased (P <0.05), while the number of colonies of intestinal bacteria have no effect(P>0.05), the sbove results shows that the gavage treatment of smoke condensate have damaging effect onthe balance of intestinal flora and oral bacteria., the treatment of passive smoking only have damaging effect on the balance of oral flora instead of intestinal bacteria.(2) The addition of smoke condensate in medium can inhibit the quantity of EPS, IPS and lactic acidproduced by intestinal probiotics (bifidobacteri and lactobacilli)(P <0.05), has little effect on EPS, IPSand lactic acid produced by intestinal pathogenic bacteria (Escherichia coli)(P>0.05), increase thequantity of EPS and lactic acid produced by S. mutans(P <0.05), large doses of smoke extract can inhibitthe IPS produced by S. mutans (P <0.05). Which explains the effects of smoke extract on the number ofcolonies in intestinal and oral bacteria from the thallus vitro products.(3) Compared with diabetic model group, The blood glucose levels has no significant difference in lowdose smoke extract group rats (P>0.05), but was significantly increased (P <0.05) in high dose of smokeextract rats; The total cholesterol level was no significant difference in low dose of smoke extract grouprats (P>0.05) but was significantly increased in high dose level of smoke extract group rats (P <0.05); Thetriglyceride, low density lipoprotein cholesterol, atherogenic index, alanine aminotransferase and aspartateaminotransferase were significantly increased (P <0.05) in smoke extract group rats, high densitylipoprotein cholesterol was decreased significantly (P <0.05). In addition, compared with diabetic modelgroup, blood glucose and blood lipid level of Stropharia Rugoso-annulata polysaccharides group weresignificantly decreased (P <0.05). This means that the smoke extract can cause a worsening of diabetes, andStropharia Rugoso-annulata polysaccharides have some role in prevention and treatment of diabetesmellitus.