| Stanniocalcin-1(STC-1) is an important regulatory glycoprotein hormone, released by Corpuscles of Stannius (CS) of bony fish and secrete into serum. Later scientists find that this protein also widely exist in mammals and human beings. STC-1involved in calcium and phosphate metabolize mainly inhibit calcium absorption and phosphate excretion and reabsorption to maintain stable calcium concentration in blood in fish. Though STC-1gene is widespread in mammals, little is known about its function. So from tissue and molecule level, we explore the expression of STC-1in gastrointestinal tract of new born cattle, which has a great significance in the research of cow calcium and phosphate metabolic disease, and give a new method to prevent and cure this disease.The research about STC-1in cow mostly fasten on gestation and lactation in blood as well as kidney and ovary. While there still no data of STC-1in gastrointestinal tract of newborn cattle and STC-1originally act as a calcium regulatory factor in kidney, gill and intestine in fish. The growth of dairy cows is a vigorous phase in calcium and phosphate absorption and skeletal development. On these bases, our study was for the fist time designed to investigate the expression of STC-1in cow gastrointestinal tract. Our purpose is to verify whether STC-1is present in cow gastrointestinal tract and which segment has the vast STC-1expression, and to provide experiment evidence for further study of STC-1in the role of calcium and phosphate homeostasis.In this study, we chose1day age newborn cow as experiment animal using In situ hybridization (ISH) and Immunocytochemistry(ICC) from tissue level to investigate STC-1expression. In situ hybridization (ISH) result showed that STC-1mRNA was expressed in rumen lamina propria, which can see dot brown stain; network edge of gastric mucosa epithelium in reticulum, the junction of gastric mucosal epithelium and lamina propria of omasum, abomasum gastric pit and fundic glands have visible granular brown staining; jejunum mucosa, intestinal absorptive cells and epithelial cells of ileum mucosa showed weak hybridization signal; mucosal epithelium and goblet cells of cecal surrounded by tan response; colonic mucosa epithelium edge absorption cells can see visible orange-brown staining. The results of immunohistochemistry showed that rumen, reticulum and omasum in the gastric mucosa layer diffuse yellow stain, dyeing near the lamina propria becomes shallow gradually. Rennet, gastric mucosal epithelial cells, gastric pit and absorbed jejunum epithelial cells, secretion of intestinal cells can see obvious plaques brown staining. Edge of ileum mucosa epithelial cells, cecum and colon mucosa epithelial cells, adenomas mucous membrane epithelial cells have obvious absorption tan granular staining. ELISA result shows STC-1protein exist in gastrointestinal tract at10-7pg level, omasum contained the largest percentage of STC-1among gastrointestinal of dissoluble protein, about24.72×10-7%and ileum is the second, at the percentage of23.53×10-7%, reticulum contain the lowest STC-1protein, bout13.24×10-7%. Little differences is on STC-1protein content in the gastrointestinal tract. Realtime PCR analysis revealed that STC-1mRNA was widely expressed in gastrointestinal tract. Duodenum and colon has the highest STC-1expression ratio, about85%and78%of the kidney. The difference of STC-1mRNA expression between jejunum, ileum and cecum is not big. At four stomach of cows, STC-1mRNA expression in omasum is higher, about.49%of the kidney. The amount of STC-1mRNA expression in the abomasum is the lowest, about17%of the kidney.In this experiment, we proved that STC-1gene expressed in gastrointestinal tract of the Chinese Holstein cows, and provides the experimental basis of STC-1express distribution and function analysis, also provide new information for the further research on STC-1physiological functions such as calcium and phosphorus metabolism in the gastrointestinal tract and absorption fatty acids. |
PDF Full Text Request