Effects Of Alpha Lipoic Acid, Beta Caro-Tene And Two Vitamins On Cryopreser-Vation Of Boar Spematozoa

Boar spermatozoa is very sensitive to temperature changes, and vulnerable to cold shock, thus lead to a low survival rate after cryopreservation for artificial insemination.Due to its unsatisfactory performance in livestock reproduction, it is hardly for cryopreservation of boar sperm to get widely used in animal husbandry. Studies have shown that oxidative damage during the freezing process is an important reason leading to the decline in boar sperm quality, and addition of antioxidants in the freezing diluent can effectively alleviate the oxidative damage and improve the quality of frozen-thawed spermatozoa. In this research, as the re-search objects, four antioxidants:alpha-lipoic acid, vitamin C, beta-carotene and vitamin E were added to the basic freezing diluent, and their effects to the quality of boar sprmatozoa after frozen-thawed in cryopreservation were explored. The main results of this research were as follows:1.Certain concentration of both alpha-lipoic acid and vitamin C added separately to the basic freezing diluent for boar spermatozoa could effectively improve the sperm viability, sperm acrosome integrity rate, plasma membrane integrity rate and mitochondrial activity of boar spermatozoa after frozen-thawed. The best results were obtained by the group adding6.0mg/L alpha-lipoic acid and the group adding400mg/L vitamin C. Compared with the control group, the difference was significant (P<0.05)2.Compared with adding individual, combination of the two applications could signifi-cantly improve the sperm viability, sperm acrosome integrity rate, plasma membrane integrity rate and mitochondrial activity of boar spermatozoa after frozen-thawed. The concentrations of the best compatibility were4.0mg/L alpha-lipoic acid and600mg/L vitamin C (P <0.05).3Certain concentration of both beta-carotene and vitamin E added separately to the basic freezing diluent for boar spermatozoa could effectively improve the sperm viability, sperm acrosome integrity rate, plasma membrane integrity rate and mitochondrial activity of boar spermatozoa after frozen-thawed. The best results were obtained by the group adding20mg/L beta-carotene and the group adding600mg/L vitamin E. Compared with the control groups, the difference was significant (P<0.05)4.Compared with adding individual, combination of the two applications could signifi-cantly improve the sperm viability, sperm acrosome integrity rate, plasma membrane integrity rate and mitochondrial activity of boar spermatozoa after frozen-thawed. The concentrations of the best compatibility were20mg/L beta-carotene and400mg/L vitamin E (P<0.05)..5. After balanced and frozen-thawed, the total antioxidant capacity (T-AOC), SOD activ-ity and GSH-Px activity of boar spermatozoa decreased significantly.6. Certain concentration of both alpha-lipoic acid and vitamin C added separately to the basic freezing diluent for boar spermatozoa could effectively improve the total antioxidant capacity (T-AOC), SOD activity and GSH-Px activity of boar spermatozoa after fro-zen-thawed. The best results were obtained by the group adding6.0mg/L alpha-lipoic acid and the group adding400mg/L vitamin C. Compared with the control group, the difference was significant (P<0.05).Compared with adding individual, combination of the two appli-cations could significantly improve the total antioxidant capacity (T-AOC), SOD activity and GSH-Px activity of boar spermatozoa after frozen-thawed. The concentrations of the best compatibility were4.0mg/L alpha-lipoic acid and600mg/L vitamin C (P<0.05).7. Certain concentration of both beta-carotene and vitamin E added separately to the basic freezing diluent for boar spermatozoa could effectively improve the total antioxidant capacity (T-AOC), SOD activity and GSH-Px activity of boar spermatozoa after fro-zen-thawed. The best results were obtained by the group adding20mg/L beta-carotene and the group adding600mg/L vitamin E. Compared with the control groups, the difference was significant (P<0.05).Compared with adding individual, combination of the two applications could significantly improve the total antioxidant capacity (T-AOC), SOD activity and GSH-Px activity of boar spermatozoa after frozen-thawed. The concentrations of the best compatibility were20mg/L beta-carotene and400mg/L vitamin E (P<0.05).