Clinical Pathology Study And Detection Of Infectious Bronchitis Virus By Isolates From Shanxi

Background:Avian Infectious Bronchitis(IB) is a kind of Acute, highly contagious disease, caused by the avian infectious bronchitis virus(IBV). It was the first described in USA in the1930s, now IB has detected in many countries around the world. The high incidence of the disease can be acute outbreaks that make young chickens dead. Adult laying hens infection can cause the yield and quality of eggs declined significantly, the disease has become one of the poultry industry harm viral diseases, which is the Office International for animal health(OIE) and country requirements poultry B/type Ⅱ infectious diseases.Methods:From2006to2009, the laboratory separate IBV variant strain named IBV SX9from suspected IBV collected from Shanxi various places. Immune chick embryo rejuvenescence virus and account EID50. Artificially infected chicks Copy artificial cases, after infect toxic1day,2day,4day,5day,7day,9day,11day,14day,21day to describe clinical symptoms, pathological changes, to detect whether have antibody in different tissues by Immunohistochemistry, setup the indirect ELISA method for this virus strains, and detected antibody titers in vivo growth and decline along with time. At different times after infected toxic, used Real-time PCR to analysis changes of viral load in chickens’ trachea, lung and kidney, understand the trend of the virus in different tissues in the body, to provide some reference information for the effective prevention and control of IBV diseases caused, to provide a theoretical basis for IBV vaccine preparation.Conclusions:The IBV SX9virus continuous passage for three times, calculate the virus EID50=10-5.3/0.1ml. Each passage the chick embryo deaths increased, and the chick embryo grow slowly, bleeding and stunting. Artificially infected the1-day-old SPF chickens through intranasally eye will lead to its lack of energy, drooping wings, drowsiness, difficulty in breathing, coughing and other symptoms. Pathological visible tracheal has catarrhal serous exudate, kidney swelling large pale, tubular, ureter filled urate typical "piebald kidney". The result of Immunohistochemistry show there have positive product in different tissues. The square tests determine the indirect ELISA antigen optimal dilution of1:4, serum dilution is1:20. The best HRP secondary antibody diluted is1:3000. According to the2-△△CT calculated the relative content of the virus. The virus content in trachea, lungs and kidney began to increase from the fourth day, the amount of virus in the kidney was significantly higher than trachea and lungs on the fourth day, the lung virus content of up to100on the seventh day, is far greater than trachea and lung, on the ninth day they all reached a peak period, the kidneys even up to150, then gradually decrease and the amount of virus is also reduced.