| Apple ring rot (Botryosphaeria dothidea) is one of the important problems ofapple, chemical control is still dominant, resistance of worthy of attention. Thisstudy by measuring, ultraviolet radiation, electromagnetic waves and salicylic acidtreatment e listed on apple’s ring rot change of different strains of drug resistant,the apple ring rot of amyl alcohol resistance law; Through the study of β-tubulinand transcription factor genes, providing theoretical basis for apple ring rot funguspathogenesis; To combine the traditional research method and molecular biology,studies the sensitivity of the apple ring rot, the construction of phylogenetic trees,clear apple ring rot fungus of amyl alcohol resistant genetic differentiation. Theresults are as follows:1Collection and separation of different apple ring rot of apple productionstrains, different ways induction treatment, the use of tebuconazole mycelial growthinhibition rate of probability values and drug concentration regression analysis toobtain strains EC50values measured the sensitivity of the various strains oftebuconazole. The results showed that Different the Apple Physalospora strainshave different sensitivity to tebuconazole, the same strain after different treatmentsthere are differences in sensitivity to tebuconazole. Continuous used oftebuconazole caused most of Apple Physalospora strain the emergen ofdrug-resistant, resistant to strengthen the increased usage make，0.1mg/L,1mg/Land10mg/L will make strains resistant to change; the ultraviolet each treatment hasa significant impact Apple piricola resistant, but the intensity of the differenttreatment markedly different, bacteria resistance to change and the processing timeis not a positive correlation,253.7nm uv processing1minute,5minutes and10minutes, strain resistance has both increased and decreased; also has a significantimpact of electromagnetic waves processing the Apple piricola the resistant bacteriaresistance to change and the processing time is not a positive correlation,700MHZelectromagnetic treatment for10seconds,40and160seconds, the weakened strainof drug resistance are enhanced; salicylic acid treatment on apple ring rot bacteriathere is an apparent resistance, but there are significant differences in the intensityof different concentrations.0.01mg/L,0.1mg/L and1mg/L of salicylic acid havedifferent influence on strain.. 2Field experiment to determine processing strain pathogenicity of applebranches,the results show that different treatment, different strains pathogenic forcehave obvious difference, one of the strongest pathogenicity strains is700MHZelectromagnetic processing of40seconds from the shandong haiyang YT1strains,the inoculated apple branches of disease index of70.37; The weakest form of strainis253.7nm treatment5minutes from shandong qixia YT1and0.1mg/L mediumcultivation e listed eight generations from henan zhengzhou ZZ42strains, theinoculated apple branches disease index of13.58; This shows that the differenttreatment of different strains of different virulence. Found that there was asignificant difference in disease index.3Using a spectrophotometer on the the Original collection strains and thestrains treated in the ultraviolet region, visible and infrared regions, respectively,with a350nm,650nm and950nm wavelength absorbance values measured strainsmetabolism solution. To the same strain in the determination of absorbance valuesunder different process,Detection and analysis of the results, different treatmentswill result in the difference of strains in the the metabolic solution absorbancevalue.4Gene sequence analysis was performed using the CTAB method strains oftotal genomic DNA using primers ITS1and ITS4the amplified ITS clip, EF-1alphagene fragment was amplified using primers728F and986R. Showed that the thethe Apple Acid.Chlorophyll rDNA ITS gene Botryosphaeria dothidea ITS genesequence similarity up to99%the the Apple rDNA of Physalospora bacteria EFα-1gene Botryosphaeria dothidea EF-1alpha gene sequence similarity up to98%,Apple wheel pattern bacteria the rDNA beta-tubulin gene and the Botryosphaeriadothidea beta-tubulin gene sequence similarity of99%. ITS gene construct theApple Acid.Chlorophyll with other closely related bacteria, EF-1alpha gene andbeta-tubulin gene phylogenetic tree, found the Apple Acid.ChlorophyllBotryosphaeria dothidea into a separate branch, indicating that the Botryosphaeriadothidea can lead to apple ring rot The occurrence. Mutated gene under theinfluence of external factors, but the EF-1alpha gene and beta-tubulin gene retainsexist. ITS gene before and after the treatment of the same strain the sequencingcomparison and found that the sequence of ITS changes. |
PDF Full Text Request