PCR Detection And Genetic Diversity Analysis Of Solanaceae Vegetables Ralstonia Solanacearum

Bacterial wilt caused by Ralstonia solanacearum is one of the most destructive soil-borne diseases.Due to bacterial wilt has abundant genetic diversity within species and obvious pathogenicitydifferentiation, which brings a lot of difficulties to the accurate diagnosis and prevention of this disease,and there is currently no effective solution. Therefore, rapid diagnosis technique and genetic diversityanalysis of R. solanacearum of great theory significance for the breeding of resistant cultivars andintegrated management in the future.This study was obtained from47bacterial strains on the eggplant, tomato, pepper crops fromHainan province, in which12strains are in different areas of Hainan province,35strains are in a pieceof land. Using the specific primer pair Y2/OLI1, the detached bacterial wilt isolates were detected byPCR, while the16S rDNA fragments of these strains were sequenced. The results show that about300-bp specific fragment was amplified from all the detached strains of R.solanacearum isolated fromeggplant, tomato, pepper crops. The results of PCR detection and the sequencing result of16S rDNAfragment are identical, confirming the PCR assay is a rapid and specific detection method for theidentification of R.solanacearum of solanaceae vegetables.The genetic diversity of the47bacterial strains was analyzed using the three universal primer pairsREPIR-I/REP2-I, ERICIR/ERIC2and BOXA1R. fingerprinting analyses showed that high abundantpolymorphism and genetic diversity occurred not only in isolates from different areas, but also thosefrom the same field. Compared with the other two primers, the BOXA1R primer generated a little morepolymorphism. The high genetic diversity among these strains indicated that R. solanacearum hascomplex and multiple population stuctures, implying that it may have a high ability of genetic variationunder stress conditions.