Detection Of Dwarf Gene And Powdery Mildew Resistance Gene Using Molecular Marker In Wheat

High yield, high quality, disease resistance and general adaptability are the main aims of wheat breeding in present stage. To understand the distribution of dwarfing gene and powdery mildew resistance gene in wheat breeding backbone parental varieties, we detected Rhtl, Rht2and Rht8gene of257wheat breeding varieties and Pm21, Pm30, and Pm34gene of96wheat breeding varieties using STS, SCAR and SSR markers, which could provide important information for wheat lodging and powdery mildew resistance breeding. The results were as follows:(1) In257wheat varieties, the frequency of Rht8gene (106,41.2%) was highest, Rht2was higher (88,34.2%) and Rhtl was lowest (70,27.2%) in257wheat resources. As for gene combination,25materials (9.7%) were detected to contain Rht2and Rht8;24materials (9.3%) were detected to have Rhtl and Rht8;9materials (3.5%) were detected to carry Rhtl and Rht2; and5materials (1.9%) were detected to contain Rhtl, Rht1and Rht8. Sixty materials without above known gene type were detected (23.3%) in this study.(2) In96wheat varieties, the distribution of Pm21, Pm30, and Pm34genes and their combination were different. The frequency of Pm34gene (21,21.87%) was highest, Pm30was higher (9,9.37%), and Pm21was lowest (4,4.17%). What’s more, Jinhe9123(1,1.04%) was detected to contain Pm21and Pm30. Yi00-119(1,1.04%) was detected to have Pm30and Pm34. No materials were detected to carry Pm21and Pm34. We also did not find any materials with P21, Pm30and Pm34gene polymerization.60materials without above known resistance genes were detected (accounting for62.50%) in this paper.