The Effects Of Variations In TaCRE1and TaCKOX4on The Tiller, Grain Weight And Grain Density Of Wheat

Cytokinin is a kind of physiological hormone which is widespread in plants. It play an important role in the regulation of the plants growth and is also considered as a great influence on the crop yield and related traits. Therefore, it’s necessary to research the allelic variations in cytokinin receptor gene and cytokinin oxidase gene (TaCKOX), which are involved in the signal transduction and metabolism of CTK respectively. In this research, the allelic variations of cytokinin receptor gene(TaCRE1) associated with wheat tiller and cytokinin oxidase gene4(TaCKOX4) with grain weight and density were respectively investigated in the common wheat varieties including the collected and original materials in total. And the main results are as follows:1. The effective tiller (ET) was significantly related to all the tiller number at the developing stages before winter (TNBW), spring stage (TNSS) and jointing stage (TNJS) respectively, of which TNBW was the most obvious one that was significantly related to the effective tiller. TNBW showed a significant correlation with TNJS and the tiller number in spring, while TNJS was not significantly associated with TNSS.2. Allelic variation of TaCRE1was very significantly related to TNBW, TNJS and ET, except for TNSS. The differential analysis of tiller number and functional evaluation between TaCRE1alleles was also consistent with the correlation, which illustrated that the allelic variation of TaCRE1mainly influence TNBW, TNJS and ET.3. In our researches, allelic variations of TaCKOX4showed more significant negative correlation with thousand grain weight (TGW) than grain density (GD) among wheat genotypes. According to the analysis between alleles of TaCKOX4, TGW of wheat genotypes carrying B-type allele of TaCRE1was significantly higher than that of the genotypes with A-type allele. On the other hand, there was no obvious difference of grain density between the alleles, which indicated that allelic variations of TaCKOX4mainly influenced grain weight of common wheat.