Study On Microbial Solid State Fermentation Plant Meal

In our country, plant meal is rich in resources. However, there are various antinutrients and toxiccompounds in the vegetable protein which directly affect the animal digestive enzyme activity and reducethe rate of digestion and absorption of nutrients, the rational and efficient use in feed production.In order toimprove the solid fermentation of various plant meal of anti-nutritional factors in removal and thenutritional value of plant meal promotion, a great deal of solid fermenting tests were conducted with4lactic acid bacteria strains and6aerobic microorganism strains. The activities of protease and the content ofASP, and TA of ferment substance (FM) were determined.The results of the fermentation experiment showed that more than10%addition rate of bran couldpromote the growth of microorganism significantly during the fermentation of plant meal. The degradationefficiencies of Aspergillus oryzae MQ11.7was better while material and water ratio was5:3in CM andRM fermentation. The ANP, content of ASP and FG of CM-FM is1028u/g,5.52%, and568mg/kg.respectively. And the ANP, content of ASP of RM-FM is987u/g and6.62%, respectively. The degradationefficiencies of Aspergillus niger HQ27was better in GM and GrM fermentation. The ANP and content ofASP of GM-FM is923u/g, and5.32%, respectively. And the ANP, content of ASP of GrM-FM is345u/gand4.62%, respectively. Although Bacillus strains could degrade soybean protein betterly, the FM was toosticky to be further processed. Besides, the RR-RM of the plant meals was only about83%. Lactobacillusfermenti FL18produced most lactic acid while material and water ratio was5:4. The content of TA in CM,RM, GM and GrM-FM was4.49%,5.16%,5.01%, and5.79%, respectively.It was also confirmed that “two-step ferment process” was better than “one-step ferment process” indecomposing protein into peptide and amino acids. The content of ASP and TA of CM-FM is19.03%, and5.25%. respectively. The content of ASP and TA of RM-FM is19.43%, and5.98%. respectively. Thecontent of ASP and TA of GM-FM is23.64%, and5.14%. respectively. The content of ASP and TA ofGrM-FM is21.61%, and4.64%. respectively.The SDS-PAGE experimental results show that two-stepferment process could significantly improve the quality of FM.