The Toxin Types Dedction And Enramycin Restrain Expeirment Of Clostridium Perfringens Of Laying Hens In Areas Of China

Clostridium perfringens is also called Clostridium welchii,which is a conditionalpathogenic bacteria of spore genera.Clostridium perfringens is widely distributed and it cancause necrotizing enteritis,intestinal toxemia, bromatoxism and trauma aerogenesisgangrene.The pathogenic factor of this bacteria is exotoxin.The toxin type that had beenfound include α, β, γ, η, δ, ε, ι, θ, κ, λ, μ and α, β, ε,ι are the mainfactors.According to the exotoxin produced by Clostridium perfringens,it can be divided intofive toxin types of A, B, C, D, E.Fecal and intestinal samples were collected from31laying hens farms of14provinces,the research aimed to know the gene subtype and geneticdiversity of there strains were analyzed by multiplex-PCR.The study can provide importantinformation of Clostridium perfringens epidemic situation.To know how the enramycinrestrain Clostridium perfringens and to provide information for drug development,we useenramycin from a company.section1：isolation of Clostridium perfringens390samples of laying hens were collected from14provinces of Shandong, Hebei,Beijing, etc.40Clostridium perfringens strains were selected by culture with selectivemedium.The isolating rate of Clostridium perfringens is10.26%,and the rate is lower than theforeign reports.section2：toxin subtype analysisThe isolates were identified and genotyped by multiPlex PCR for the present of α,β2,ε,ι.βand cpe were identified and genotyped by PCR.1C type isolate was found and other isolateswere A type.The result provide important support to survey，prevention and diagnosis of.Clostridium perfringenssection3：enramycin restrain Clostridium perfringens experimentTwo differant batchs of enramycin were used to restrain the Clostridiumperfringens.0.01ug/ml of enramycin can restrain Clostridium perfringens obviously and0.01u/ml can cause the first high inhibition.From0.5ug/ml the inhibition will be srongeragain.The result of the experiment makes a contribution to further research and development of the enramycin.