| Rice blast is one of the most destructive rice diseases caused by the fungal pathogen Magnaporthe oryzae. Utilization of resistant rice cultivar against blast is the most effective strategy which is a safe, economical and environmental friendly comparing with chemical control methods. Mapping and cloning the blast resistance genes will provide important germplasm for breeding new resistant rice cultivar and help to understand the molecular interaction between rice and Magnaporthe oryzae.The rice cultivar Mowanggu is originated from Yunnan province in China, and possesses durable and broad-spectrum resistance to blast. Analysis the Recombinant Inbred Lines (RILs) F8and F9population derived from the cross between Mowanggu and the susceptible cultivar CO39indicates that there is a major R gene which located on chromosome11responsible for its resistance. On this basis, we fine mapped this gene and named Pi49. The main results are as follows:1. The Pi49mapped in the interval between SSR marker RM224and SRR marker RM144through the208susceptible individuals.2. Basing on developing new molecular markers between RM224and RM144, the Pi49was further located between the CAPS marker K27and SSR marker K10with genetic distances0.16cM and0.16cM, respectively.3. Constructed the BAC library of Mowanggu. We screened out the library with two flanking markers K10and K134by the enrichment cultivar PCR, and got the mono-clony containing the Pi49fragment. |
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