Effect Of Dark Tea Extract On The Steatosis Model And The Expression Of Lipid Metabolism-associated Gene

Dark tea is a unique kind of tea in our country, belongs to one of the six major tea, which have strong lipid-lowering effect of losing weight. however, the specific mechanism is unknown until now. And dark tea has been show the lipid-lowering effect in vitro animal experiments, but these aren’t so many research reports about the organ cells level and gene level research.This experiment using four different black tea extract——The Fu brick tea extract(FZ), Liubao tea extract(LB), Qing brick tea extract(QZ), Qianliang tea extract(QL) as the experimental material to studies the influence of lipid metabolism related genes expression.1、The establishment and optimization of the steatosis model by Fetal bovine serum lipidwhen administered conditions which is contained30%,50%,70%FBS in48h to establish the model of hepatic steatosis in vitro,MTT value, total triglyceride (TG), total cholesterol(TC) and were used to evaluate the effect of dark tea on HepG2steatosis.at last,50%FBS could bring the accumulation of fat in vivo, in which cell survival rate is high.2、Study on the effect of dark tea on steatosis modelThese dark tea were assessed for the hepatic lipid-lowering potential when administered conditions which is contained50%FBS in48h to establish the model of hepatic steatosis in vitro. MTT value, total triglyceride (TG), total cholesterol(TC) and were used to evaluate the effect of dark tea on HepG2steatosis. Moreover, FZ,LB,QZ,QL all show highly significant inhibitory effect against TG accumulation(P <0.01). FZ,QZ also significantly reduced the TC accumulation(P<0.01),however, the effect of LB and QL are obvious. These result showed dark tea extract might reduce lipid accumulation in cell. It showed the suppressive effect on lipid accumulation.3%Effect of dark tea on expression of SREBP-1c、FAS mRNA of steatosis model when these200μg/mL tea extracts were added in the treatment groups,FAS gene expression,SREBP-lc gene expression is also detected at the gene level.Compared with the model groups, the expression of SREBP-lc、FAS mRNA was significantly suppressed in the treatment groups such as FZ,LB,QZ. However, QL treatment group upgrade the expression of SREBP-1c、FAS mRNA.