Study On Wilt-resistance Of Lily

Fusarium wilt is one of the major diseases of Lily, the serious harm had a greatimpact on the quality and yield of cut lily. Currently, the most effective way to combat Lilywilt is nurturing and screening of resistant cultivars.This article first isolated LilyFusarium wilt from sick plants, and then purificate, culture and identify it. Besides, it madea research about wilt resistance on the9common market lily species (varieties);explore themain factors to affect the lily of fusarium wilt:inoculation concentration, inoculation time,inoculation temperature;explore the relation between the response of physiological andbiochemical after Lily infect disease and wilt resistance; explore the role of salicylic acidinduced Lily resistance to fusarium wilt. The main results showed as follows:(1) Using scales and detached leaves inoculation identification,9species (varieties)lilies were tested of resistance to fusarium wilt. The results showed that, there is asignificant difference on9species (varieties) lilies wilt resistance: in addition to the’Bright’ and ’Snow Queen’,remaining seven varieties are consistent by the two ways.Lilium pumilum and’ Purito’ are high-resistant varieties, L. lancifolium,’Sorbonne’ and’White Heaven’ are medial-resistant materials,’Siberia’ is susceptible and L. concolor var.buschianum.is high-susceptible material.’Bright’ is medial-resistant variety by thedetermination of detached leaves inoculated, highly resistant varieties by the scalesinoculated determination;’Snow Queen’ was measured in susceptible varieties detachedleaf inoculation method, and the determination of scales inoculation method formedial-resistant material.(2) Take the susceptible cultivar ’Siberia’ and resistant varieties ’Purito’ as test materialsto study the factors affecting the incidence of lilies,the results showed that, early leafexpansion, temperature is25℃, relative humidity of80to90%, and the concentration ofthe spore suspension of Fusarium106/mL, placed time in less than0.5h, the lilies is mostvulnerable to disease.(3) After artificial inoculation, the relation between the changes of leaf relativeconductivity, MDA content, POD, PAL,PPO activity and wilt resistance showed that:Fusarium made effects on leaves active oxygen scavenging system and Organicosmolytes of ’Purito’,’Sorbonne’ and L. concolor var: when plants were infected with thebacteria, the relative conductivity and MDA content of the leaves were increased,and the rise magnitude of resistant varieties was smaller than the susceptible varieties. The resultsof enzyme activity assays showed that: the resistant and susceptible varieties beforevaccinated Fusarium, POD, PAL,PPO activity of resistant varieties were higher thansusceptible varieties, but the difference was not significant; after the Fusarium inoculation,PAL, POD and PPO activity of the tested cultivars were all increased, and PAL, PPOactivity of resistant varieties increased significantly greater than the susceptible cultivars,but POD activity of resistant and susceptible varieties increased was not significantlydifferent, resistant varieties appears twice enzyme activity peak and susceptible varietiesonly once enzyme activity peak; lilies wilt resistance were closely related to POD, PAL andPPO activity.(4) The relationship between salicylic acid and lilies resistance to Fusarium wilt: by themethod of determination of the growth rate, Fusarium oxysporum was not been inhibitedin the concentration containing medium0.1,0.5,1.0,5.0,10mmol/L SA,SA was not directtoxicity of Fusarium. After SA induction treatment, it can cause the POD, PPO, PAL, SODactivity of the lilies leaves increase, thereby enhancing the lilies ability of resistance toFusarium wilt.