Plants Antimicrobial Protein SN1, PvPGIP2Transgenic Wheat Get,Molecules And Disease Resistance Identification

The study of plant protein gene through antibacterial PvPGIP2, SN1cloning and expression in the construction of the wheat carrier. Through the genes for marksmanship transgenic wheat, and its molecular sign and agronomic characters analysis, preliminary got WenKuBing resistance and soil spread fungus diseases of genetically modified wheat plant. The main conclusions are as follows:(1) Using homology cloning strategy and RT-PCR technology, success in cloning from potatoes to antimicrobial peptides SN1gene sequences, and with pA25skeletons carrier, to construct the pA25-SN1expression vector, using gene marksmanship, will pA25-SNl into wheat variety "Yang m18". SNI simulating genetic wheat prearcing-T2generation plants PCR, Q-RT-PCR, Southern blotting analysis and WenKuBing bacteria, root bacteria etc and its resistance identification technology inoculation, molecular testing results show that SN1gene into wheat variety "Yang m18", and can genetic, genetically modified in the expression of wheat SN1quantity are higher than the receptor control wheat variety "Yang m18". SN1upon the WenKuBing, wheat gene appeared resistance identification, were given the2kinds of disease resistance of genetically modified wheat materials.SN1proof excessive can improve the genetically modified to express wheat WenKuBing bacteria and root of bacteria resistant..(2)Using homology cloning genes strategy and RT-PCR technology, successfully cloned a string beans of half of lactose acid aldehyde together enzyme inhibition protein (PvPGIP2) coding genes, the pA25for constructing the frame PvPGIP2gene expression carrier, using gene marksmanship, will pA25-PvPGIP2into wheat variety "Yang m18". PvPGIP2simulating genetic wheat prearcing-T2generation plants PCR, Q-RT-PCR and WenKuBing bacteria resistant identification technology and inoculation, molecular testing results show that PvPGIP2gene into wheat variety "Yang m18", and can genetic, genetically modified in the expression of wheat PvPGIP2quantity are higher than the receptor control wheat variety "Yang m18". PvPGIP2upon the resistance gene wheat WenKuBing identification, get an WenKuBing harm of genetically modified wheat materials. PvPGIP2proof excessive can improve the genetically modified to express wheat WenKuBing bacteria and root of bacteria resistant.