Isolation And Identification Of Streptococcus From Swine And The Immunogenicity Analysis Of SEZ Protective Protein

swine streptococosis were caused by streptococcus suis、 streptococcus equi subsp.zooepidemicus and many other sero-group streptococcus,In addition, S. suis has also been described as an important zoonotic agent,Hunan infections through with the breakage skin or aerophore, the seriously infection may cause human dead. So far, whole-cell vaccines orbacterins (commercial and autogenous) have been used in the field to prevent S. suis disease, however, with disappointing results Vaccination with these bacterins does not induce high levels of antibodies and causes, at most, serotype-specific responses.this study separate two parts,the one is chorisis and identify the bacteria from pathological materials, where came from part of the scaled-piggeries in Hunan province, with being suspected by checking under microscope,3pairs of specific primers for three bacteria, streptococcus, S.suis and S.equi subsp.zooepidemicus, were designed, which is based on the conservation of gene sequence on16S rRNA. Another4pairs of primers, which is based on the specification of capsular polysaccharide(CPS), were also designed for detecting the presence of serotype1(14),2(1/2),7and9, which are prevalent in streptococcus suis in recent years. As a result,24strains of streptococcus suis were detected, in which contains serotype2(9strains), serotype9(4strains), and other serotypes(11strains),1strains of streptococcus equi subsp.zooepidemicus,4strains of other streptococcus and7strains of other bacteria. the results shows that many other virulent bacteria can cause the occurrence and prevalence of swine streptococosis, in addition to different serotype S.suis and S.equi subsp.zooepidemicus.The second is znuA, sp1, sp2, sp3, sell, cspZ protein gene’s cloning expression and immunogenicity analysis. Western Blot analysis and animal testing showed that all of the recombinant protein exhibited antigenicity. The result shows, znuA、 sp1、 sp3could induce both humoral immunity and cellular immunity, they are good vaccine candidate.