Ningbo, Zhoushan Port Channel Separation Of Pathogenic Bacteria And The Virulence Gene Isolation

Vibrio parahaemolyticus (of Vibrio parahaemolyticus, VP) is a Gram-negative halophilic bacteria, widespread in the estuarine and marine environments, it is not only seriously harm the marine aquaculture industry, but also lead to human gastroenteritis, food poisoning disease. Our part of the coastal regions of Vibrio parahaemolyticus food poisoning caused by bacterial food poisoning in the first place, mainly due to eating caused by the pathogen contamination or improper processing of seafood, food poisoning incidents in recent years, triggered by its endless. Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) is a major virulence factor of Vibrio parahaemolyticus, composed of165amino acids, respectively, between the homology of67%, and has the same biological activity, such as hemolytic activity, the activity of intestinal drug, cell toxicity and cardiac toxicity. Almost all clinical isolates can produce the Kanagawa phenomenon (Kanagawa phenomenon, KP), this phenomenon is related with the TDH, although environmental isolates rarely have a KP-positive, but carry the tdh or trh gene can be considered to be a virulent strain. Other Vibrio, some Vibrio parahaemolyticus clinical isolates produce urease, studies have shown that purified urease can cause neonatal rat intestinal fluid accumulation, while the ureC gene is the most stable structure of genes in the urease gene cluster, scholars believe that Vibrio parahaemolyticus urease positive and carrying trh gene and urease gene clusters with trh gene linkage.This study investigated the Vibrio parahaemolyticus in Zhoushan, Ningbo of Zhejiang coastal sea pollution, and explore the different sources of Vibrio parahaemolyticus isolates virulence genes tdh, of gyrB and the tlh.Aeromonas (of Aeromonas) bacteria are common parasites rot, is widely distributed in nature, water and soil have occasionally in the feces of healthy people can be isolated Aeromonas hydrophila, Aeromonas (of Aeromonas hydrophilia representative species of the genus wide range of aquaculture animal diseases caused by Aeromonas skin fester disease, such as silver carp and bighead carp, grass carp hemorrhagic disease, ascites disease hemolytic different carp, carp of erythema disease and vertical scales disease, eel red fin disease and perforation of turtle diseases such as the occurrence of these diseases is not only serious economic losses to the aquaculture industry, can also be caused by aquatic animals and aquatic infection to humans and animals, humans and animals from diarrhea and food poisoning, affecting human health. especially in the genus Aeromonas hydrophila, Aeromonas, the main pathogens of a variety of aquatic animals are human beings-animals-aquatic animals sick pathogens.This study investigated the pollution situation in Zhoushan, Ningbo of Zhejiang coastal sea Aeromonas, and explore the different sources of Aeromonas isolates, the major virulence gene Aerolysin gene an aer A hemolysin gene the hly A, adhesins ahalahal, cell excitability enterotoxin genes alt.Vibrio alginolyticus of Vibrio alginolyticus diarrhea pathogens in an obligate halophilic vibrio. And other pathogenic Vibrio, is widely distributed in the oceans and rivers into the waters of Haikou, and the largest number of the first of seawater class Vibrio, in the past has been considered not pathogenic or only caused by some traumatic infection without attention in recent years The study confirmed that the bacterium Vibrio parahaemolyticus, like the common pathogens, as one of the dominant strain of the marine environment. Vibrio alginolyticus can cause human food poisoning, otitis media and sepsis, summer is the main season of farmed animals alginolyticus Vibrio incidence of infection, prone to bacteria caused major epidemics, particularly in China is southern mariculture cause the huge threat. At present, China’s GB detection of Vibrio alginolyticus does not specify, explore the isolation and identification of Vibrio alginolyticus in seawater and seafood, its security controls and subsequent detection of Vibrio alginolyticus standards is of great significance.This study investigated the Vibrio alginolyticus in Zhoushan, Ningbo of Zhejiang coastal sea pollution, and explore different sources of Aeromonas strains isolated virulence genes alkaline serine protease aspA gene alginolyticus Vibrio collagen enzyme gene is valC and isolate specific genes of Vibrio alginolyticus collagenase gene.