Analysis Of Mineral Elements Content In Brown Rice Of Rice Germplasms

Mineral elements are necessities for human being and play an important role on human growth and development. Rice is one of the most important food crops in China, and the mineral nutrition in rice play a key role for consumer health. As a result, accumulation of nutritional elements in rice is the foundation for human health. In this study,533collections are the main research materials. The content of N and P in brown rice of these accessions have been determined by colorimetry. Another12elements content including B, Mg, K, Ca, Mn, Fe, Cu, Zn, Se, As, Cd and Pb have been tested using ICP-MS. We analyzed content and distribution characters of these elements in brown rice. The results obtained mainly as follows:1. The content of N, P, Mg, K, Ca in brown rice shows significant difference among the different rice collections. The order of these elements content is N>P>K>Mg>Ca and the order of their coefficient of variation is Ca>K>Mg>N>P. The content of N, P, Mg, K, Ca in brown rice conforms to normal distribution. The order of microelements in brown rice is Fe>Mn>Zn>Cu>B>Se. The microelements’ content shows significant difference among the different rice collections. We could select several collections with extreme elements content as parents to construct mapping populations or cultivate improved varieties. The content of harmful elements, such as As, Cd, Pb, in brown rice shows significant difference among the different rice collections. The Cd content in brown rice is lower than the national standard, the Pb content in brown rice close to the limit level and the As content exceeds national standard.2. We select some collections with enrich mineral nutrition in brown rice, which have higher content of N, Mg, K, Ca, Mn, Fe, Cu, Zn than the sum of mean and standard deviation. We also select collections with low harmful elements content in brown rice, which have lower content of As, Cd, Pb than national standard and the difference of mean and standard deviation. These collections could be provided for breeding as candidate materials.3. According to the results of difference analysis for mineral elements content among different subgroups, we find content of N, P, K, B, Mn, Fe, Cu, Zn in brown rice of Japonica is higher than Indica, and there is significant difference between them. The content of P, Ca, Fe, Cu, Zn in brown rice of Aus reaches a significantly higher level than Indica. The content of K, B, Mn in brown rice of Japonica is higher than Aus, but Cu and Zn content of Japonica is lower than Aus, there is significant difference between them. There is no significant difference between Cd content of Japonica and Aus, but they’re both significantly lower than Indica. The Pb content in brown rice of Indica is significantly lower than Japonica. There is no significant difference in Pb content of Aus compared with Japonica and Indica.4. According to statistics, there are458collections with white seed coat (white rice),71collections with red seed coat (red rice) and4collections with black seed coat (black rice). The content of N, P, Fe, Cu, Zn, Se, As, Pb in red rice is significantly higher than white rice. There is no significant difference in content of14mineral elements in black rice compared with white rice and red rice.5. According to correlation analysis, there is highly significant positive correlation among most macro-elements. There is also highly significant positive correlation between content of Zn and Mn, Fe and Cu, Zn and Cu. The Zn content is very significantly positive related to content of Mg, K and Ca. It presents significant positive correlation between Mn and K, Ca content. Fe, Cu and Se don’t highly relate to macro-elements. There is highly significant positive correlation between As and Pb, and remarkable negative correlation between As and Cd. According to correlation analysis between mineral elements and harmful elements, As content highly significantly positive relates to content of Mg, Ca and Fe, and Pb content highly significantly positive relates to content of Fe and Cu. There is no significant correlation between Cd and other mineral elements.