| In recent years, false smut (caused by U.virens) has risen as one of the main diseases of rice. Sterol14a-demethylase (cyp51) plays an important role in sterol biosynthesis which is an critical antifungal target. Triazole compounds as14a-demethylase inhibitors (DMIS) are widely used in the production and daily life, however, due to their single targets led to the frequent occurrence of resistant strains. Therefore, the study of U. virens CYP51sequence and structure as well as their interaction with fungicides mechanism is very significant to design the specific, low-toxic, high effective DMIs.This paper firstly revealed the sequence and structure of U. virens CYP51, and analyzed the characteristics of its interaction with Triazole drugs. The results would provide theoretical basis for the development of effective fungicides.On the one hand, the currently known CYP51sequences were alignmented.The CYP51F2gene of U. virens were successfully cloned by using specific primers, which full-length is1551bp encoding516amino acids. Degenerate primers for CYP51F1of U. virens were designed, and the partial fragments of cyp51F1were amplified by using nested-PCR. According to morphological and molecular identification, we speculate that there may be a phenomenon of horizontal gene transfer between CYP51gene of U. virens and rice blast fungus.On the other hand, the sensitivity of U. virens to the Triazole drugs were analyzed. Toxieity determination results showed that propiconazole is highest toxieity, its IC50=0.0377mg/L, Tebuconazole IC50=0.1002mg/L, diniconazole IC50=0.2047mg/L, Triadimenol IC50=0.4431mg/L, and Triadimefon IC50=2.9038mg/L. To further, the characteristics of U. virens cyp51interaction with propiconazole was studied according to molecular docking. The results showed that there are8amino acids may be the critical of binding to the propiconazole. The π-π conjugation and Hydrophobic interaction are the most important forces. |
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