Analysis Of Agronomic Traits In Transgenic Rice Lines Of CCAAT Transcription Factor Family

CCAAT transcription factor belongs to the basic leucine zipper family andplays an important role in cell differentiation, energy metabolism, growth anddevelopment. The CCAAT family of rice contains46different gene sequences,however, only a few of genes have been identified. In this study, we cloned CCAATtranscription factor genes from rice and attached to the over-expression vector byGatewayTMcloning technology. We transferred the vectors into rice callus withagrobacterium mediated transformation method and obtained those transgenic plants.We analyzed agronomic traits of T2transgenic lines in different locations, providedinformation about agronomic performance of CCAAT family genes.The main results were summarized as follows:1．Cloning and transformation of CCAAT family genesThe japonica rice variety, Nipponbare, was used as the material. Thirty-sixCCAAT family genes had been cloned and constructed to the transcription activateexpression vector (VP64-pCambia1301-UbiN) and the transcription inhibitexpression vector (EAR-pCambia1301-UbiN) by GatewayTMtechnology,respectively. We transferred the vectors into Kitaake using agrobacterium mediatedtransformation method. A total of513T0transgenic lines were gained in17CCAATfamily genes.2．The selection of excellent lines using T1transgenic rice linesAccording to the results of yield performance, four genes LOC_Os01g24460.1,LOC_Os01g70880.1, LOC_Os02g49370.1, and LOC_Os08g09690.1were selectedin T1lines, and a total of12lines were used as subsequent test.3．The analysis of agronomic traits in T2transgenic rice lines According to the difference significance analysis of7agronomic traits in T2transgenic rice lines, the agronomic traits of transgenic rice were mainly affected byplant height, full grains per panicle and yield per plant in Changchun, Jilin andLingshui, Hainan. The three traits were showed high significant differences contrastwith wild type (Kitaake). Highly significant correlation was found between spikesper plant, full grains per panicle and yield per plant of transgenic rice in differentlocations.4．The molecular biological identification of transgenic ricePCR amplification and sequencing analysis of CCAAT family genes showedthat the genes integrating into the rice genome. Furthermore, The PCR identificationof Hpt gene also proved above conclusion.