Agricultural Probiotics Screening Compound

Living microbial fertilizer is fertilizer, which mainly depends on a lot of beneficial microorganisms, using its strong reproduction and metabolism, the physical transformation and the formation of beneficial metabolites to continue. Microorganism The microorganisms with nitrogen fixation, Phosphate, Potassium solution. The role of microorganisms in agriculture has gradually been recognized, Therefore, the types of beneficial microorganisms in the microbial fertilizer, whether the activities of life strong is the basis of their effectiveness, rather than the other fertilizer is nitrogen, phosphorus, potassium and other elements in the form and how much. Microbial fertilizers living preparations, so closely related to the fertilizer and the number of viable cells, the intensity and the surrounding environmental conditions, including temperature, moisture, pH, nutrient conditions and the original life of indigenous microorganisms in the soil repulsion to have some impact. Microbial fertilizer production in China achieved economic and social effects, and gradually the industrial production stage, the production of microbial fertilizer in China has achieved some economic and social effects, and gradually moving towards industrial production.In this study, taken from the vegetable soil, according to10-1,10-2,10-3,10-4,10-5,10-6,10-7dilution and inorganic phosphorus coating to the plate medium, nitrogenase Medium plates, potassium medium plate, at28℃under the conditions of inorganic phosphorus from the solid medium, medium potassium, nitrogen-fixing medium were initially screened a P1solubilizing bacteria as fungi, five solutions K strain Kl, K2, K3, K4, K5, in which K1, K2, K3is a bacteria, K4, K5is the actinomycetes, two nitrogen-fixing bacteria N1,N2. And morphology of these strains were the microscope and electron microscope.In this study, the key technology isolated from the vegetable soil with solubilizing solution of potassium ability of microorganisms and the optimization of training to enhance the degradation ability.The main theoretical basis for the determination of potassium capacity through the use of flame spectrophotometric determination of potassium content; The theoretical basis for the determination of phosphate solubilizing capacity plate method, ie, determination of the size of the circle of the microbial growth in defined medium. In addition, the experiments by optimization of culture conditions, the degradation of the original strains of phosphorus and potassium. After rescreening the solution of potassium ability the K2, the nitrogen-fixing bacteria N1and phosphate-solubilizing bacteria growth curve drawing, and explore the growth pattern of the three bacteria.And three strains were mixed, the results show that the mixed solution of potassium bacteria and phosphate-solubilizing bacteria still has a strong degradation ability.